Genetic difference between Ezo-awabi Haliotis discus hannai and Kuro-awabi H. discus discus populations: Microsatellite-based population analysis in Japanese abalone

Motoyuki Hara, Masashi Sekino

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

This study documents the genetic population relationships between Ezo-awabi Haliotis discus hannai and Kuro-awabi H. discus discus by means of the microsatellite technique with an emphasis on the extent of genetic difference between Ezo- and Kuro-awabi populations. Eight markers were employed to screen five populations each collected from Ezo- and Kuro-awabi habitats. All eight loci showed polymorphisms in all populations (number of alleles per locus, 11.1-14.1; averaged expected heterozygosity, 0.64-0.70). The number of loci that yielded significant genetic heterogeneities (allele frequency distribution and/or pairwise θ) between Ezo- and Kuro-awabi populations was larger than that generated in comparisons between populations within Ezo- and Kuro-awabi habitats. According to the bootstrap neighbor-joining trees constructed on the basis of two genetic distance measures (DA and DST), the 10 populations fell into two clusters of populations (Ezo-awabi and Kuro-awabi habitat groups), and the results of hierarchical AMOVA testing also supported the neighbor-joining clustering. The outcomes presented here suggest that the microsatellite markers used in this study are potentially an efficient means to differentiate Ezo- and Kuro-awabi populations.

Original languageEnglish
Pages (from-to)754-766
Number of pages13
JournalFisheries Science
Volume71
Issue number4
DOIs
Publication statusPublished - 2005 Aug 1
Externally publishedYes

Keywords

  • Genetic relationship
  • Haliotis discus discus
  • Haliotis discus hannai
  • Japanese abalone
  • Microsatellite DNA
  • Population genetics

ASJC Scopus subject areas

  • Aquatic Science

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