Genetic diagnosis of cancer by using yeast

T. Suzuki, Chikashi Ishioka, R. Kanamaru

Research output: Contribution to journalArticlepeer-review

Abstract

Current progression of molecular genetics and cellular biology permits genetic diagnosis of cancer, including the detection of both germline and somatic mutations in a number of oncogenes and tumor suppressor genes. Structure-based methods, such as PCR-SSCP and PCR-DGGE, are usually used for the first genetic screening. Although these methods efficiently detect most structural changes in DNA, they are unable to distinguish between mutations and polymorphisms. Since mutations that affect functions of gene products are only important for genetic diagnosis, we developed yeast-based methods that are able to detect loss-of-function mutations of tumor suppressor genes. In this review paper, we introduce two representative assays using Saccharomyces cerevisiae, functional assay for the p53 gene (FASAY) and stop codon assay (SC assay) for the APC gene and other tumor suppressor genes.

Original languageEnglish
Pages (from-to)1177-1182
Number of pages6
JournalBiotherapy
Volume11
Issue number11
Publication statusPublished - 1997 Dec 1

Keywords

  • APC
  • Functional assay
  • Stop codon assay
  • Yeast
  • p53

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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