Abstract
Objective. To clarify the mode of inheritance of the tissue distribution of vasculitis in MRL/Mp.lpr/lpr (MRL/lpr) lupus-prone mice and to identify the susceptibility loci. Methods. Vasculitis in individual MRL/lpr, C3H/ HeJ-lpr/lpr (C3H/lpr), (MRL/lpr × C3H/lpr)F1, and (MRL/lpr × C3H/lpr)F2 intercross mice was analyzed by histopathologic grading of main branches of the aorta and of medium-sized arteries in the lower limbs. Genomic DNA samples from F2 intercross mice were examined by simple sequence-length polymorphism analysis, and the polymorphic microsatellite markers highly associated with vasculitis in each tissue were determined as vasculitis susceptibility loci. Results. A susceptibility locus with significant linkage to vasculitis of main branches of the aorta was mapped on chromosome 4 at D4Mit213 (map position 13.3cM) selectively in males, while vasculitis of mediumsized arteries in the lower limbs was mapped to different chromosomes: at D8Mit31 on chromosome 8 (map position 33.0) selectively in females and at D5Mit36 on chromosome 5 (map position 65.0). All of these were different from the previously defined loci governing susceptibility to vasculitis involving the kidneys. Conclusion. Systemic vasculitis in MRL/lpr mice is genetically controlled with cumulative effects of multiple gene loci, each of which has tissue specificity.
Original language | English |
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Pages (from-to) | 1445-1451 |
Number of pages | 7 |
Journal | Arthritis and Rheumatism |
Volume | 48 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2003 May 1 |
ASJC Scopus subject areas
- Immunology and Allergy
- Rheumatology
- Immunology
- Pharmacology (medical)