Genetic and physical maps and a clone bank of mitochondrial DNA from rice

M. Iwahashi, M. Nakazono, A. Kanno, K. Sugino, T. Ishibashi, A. Hirai

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Mitochondrial DNA (mtDNA) was isolated from young green leaves of rice plants. DNA fragments were cloned into lambda DNA, and clones that hybridized to mitochondrial genes from other plants were selected. Distal restriction fragments of these clones were used as probes for the selection of overlapping clones. A genetic map was finally created from the library by "walking" along the genome. The mitochondrial genome consists of five basic circles, with each circle sharing homologous sequences with one or two other circles. A master circle was constructed from the results of recombination across repeated sequences, and its size was estimated to be 492 kb. A physical map and a bank of overlapping clones were also constructed.

Original languageEnglish
Pages (from-to)275-279
Number of pages5
JournalTheoretical and Applied Genetics
Volume84
Issue number3-4
DOIs
Publication statusPublished - 1992 Jul 1
Externally publishedYes

Keywords

  • Clone bank
  • Genetic map
  • Mitochondrial DNA
  • Physical map
  • Rice (Oryza sativa)

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

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