Genetic and physical mapping of genes involved in pyoverdin production in Pseudomonas aeruginosa PAO

M. Tsuda, H. Miyazaki, T. Nakazawa

    Research output: Contribution to journalArticlepeer-review

    45 Citations (Scopus)

    Abstract

    Pseudomonas aeruginosa PAO was mutagenized with Tn1737KH, a type I transcription probe transposon containing a promoterless lacZ (β- galactosidase) gene, and 24 insertion mutants that did not grow under iron- deficient conditions were isolated. None of the culture supernatants from any mutants contained pyoverdin, a low-molecular-weight siderophore able to sequester ferric iron at very high affinity, and the growth defects of the mutants were all phenotypically recovered by the addition of the culture supernatant from the wild-type strain. These phenotypes led to the inference that all the mutants had defects in the genes (pvd genes) for production of pyoverdin. In some pvd::Tn1737KH mutants, high levels of β-galactosidase activities were observed, and such activities were drastically reduced by the addition of ferric ion in the culture media, indicating that the expression of at least some pvd genes is regulated at the transcriptional level. Molecular cloning and physical analysis of the chromosomal fragments with Tn1737KH insertions allowed us to allocate all the mutations within a 103-kb region, referred to as the pvd region, that was found to locate at 47 min on the genetic map of PAO. Further physical mapping and Southern analysis showed that there is a 10-kb overlap between the pvd region and the 125-kb catA region described by Zhang and Holloway (C. Zhang and B. W. Holloway, J. Gen. Microbiol. 138:1097-1107, 1992). We could hence illustrate the physical map of the P. aeruginosa chromosome with a size of 218 kb.

    Original languageEnglish
    Pages (from-to)423-431
    Number of pages9
    JournalJournal of bacteriology
    Volume177
    Issue number2
    DOIs
    Publication statusPublished - 1995

    ASJC Scopus subject areas

    • Microbiology
    • Molecular Biology

    Fingerprint Dive into the research topics of 'Genetic and physical mapping of genes involved in pyoverdin production in Pseudomonas aeruginosa PAO'. Together they form a unique fingerprint.

    Cite this