Genetic analysis of thiopurine methyltransferase polymorphism in a Japanese population

Masahiro Hiratsuka, Tomoko Inoue, Fumiko Omori, Yasuyuki Agatsuma, Michinao Mizugaki

Research output: Contribution to journalArticlepeer-review

78 Citations (Scopus)

Abstract

Thiopurine methyltransferase (TPMT) catalyses the S-methylation of thiopurine drugs such as 6-mercaptopurine, 6-thioguanine, and azathiopurine. Several mutations in the TPMT gene have been identified which correlate with a low activity phenotype. The molecular basis for the genetic polymorphism of TPMT has been established for European Caucasians, African-Americans, Southwest Asians and Chinese, but it remains to be elucidated in Japanese populations. The frequency of the four allelic variants of the TPMT gene, TPMT* 2 (G238C), TPMT* 3A (G460A and A719G), TPMT* 3B (G460A) and TPMT* 3C (A719G) were determined in Japanese samples (n = 192) using polymerase chain reaction (PCR)-RFLP and allele-specific PCR-based assays. TPMT* 3C was found in 0.8% of the samples (three heterozygotes). The TPMT* 2, TPMT* 3A and TPMT* 3B alleles were not detected in any of the samples analyzed. This study provides the first analysis of TPMT mutant allele frequency in a sample of Japanese population and indicates that TPMT* 3C is the most common allele in Japanese subjects. (C) 2000 Published by Elsevier Science B.V.

Original languageEnglish
Pages (from-to)91-95
Number of pages5
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume448
Issue number1
DOIs
Publication statusPublished - 2000 Mar 14

Keywords

  • Genetic polymorphism
  • Pharmacogenetics
  • Thiopurine methyltransferase

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Health, Toxicology and Mutagenesis

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