TY - GEN
T1 - Generation of sub-nanosecond 586-nm optical pulses based on the synchronously gain-switched laser diodes with optical injection locking
AU - Hung, Jui Hung
AU - Gao, Yuan
AU - Yan, Hejie
AU - Sato, Kazuo
AU - Yamada, Hirohito
AU - Peng, Lung Han
AU - Yokoyama, Hiroyuki
PY - 2019/6
Y1 - 2019/6
N2 - Stimulated emission depletion (STED) technology incorporated with fluorescence microscopy envisions biomedical samples to sub-diffraction-limit resolution. Pulsed-mode STED microscopy is attractive because milliwatt-level low average optical power can induce notable STED effect [1]. However, it is difficult to find stable and compact optical pulse sources that are suitable for STED. The pulsed operation of a laser diode (LD) can provide the stability and compactness, but the generated optical power is not very sufficient in general [2]. Therefore, we previously demonstrated a 1.3 μm LD-based optical pulse source using an optical fiber amplifier (OFA) and second harmonic generation (SHG) to obtain high peak power (and thus high pulse energy) at 650nm band [3]. However, when we want to generate optical pulses in yellow-orange spectral range for green fluorescent protein (GFP) and yellow fluorescent protein (YFP) labelers, we find it difficult to obtain conventional OFAs at the corresponding fundamental wavelengths for SHG. In this report, we describe a novel method to generate smooth-shaped, sub-nanosecond, 586nm optical pulse sources toward STED application.
AB - Stimulated emission depletion (STED) technology incorporated with fluorescence microscopy envisions biomedical samples to sub-diffraction-limit resolution. Pulsed-mode STED microscopy is attractive because milliwatt-level low average optical power can induce notable STED effect [1]. However, it is difficult to find stable and compact optical pulse sources that are suitable for STED. The pulsed operation of a laser diode (LD) can provide the stability and compactness, but the generated optical power is not very sufficient in general [2]. Therefore, we previously demonstrated a 1.3 μm LD-based optical pulse source using an optical fiber amplifier (OFA) and second harmonic generation (SHG) to obtain high peak power (and thus high pulse energy) at 650nm band [3]. However, when we want to generate optical pulses in yellow-orange spectral range for green fluorescent protein (GFP) and yellow fluorescent protein (YFP) labelers, we find it difficult to obtain conventional OFAs at the corresponding fundamental wavelengths for SHG. In this report, we describe a novel method to generate smooth-shaped, sub-nanosecond, 586nm optical pulse sources toward STED application.
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U2 - 10.1109/CLEOE-EQEC.2019.8871901
DO - 10.1109/CLEOE-EQEC.2019.8871901
M3 - Conference contribution
AN - SCOPUS:85074641606
T3 - 2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2019
BT - 2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2019
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2019
Y2 - 23 June 2019 through 27 June 2019
ER -