TY - GEN
T1 - Generation of patterned cell co-cultures inside tubular structure using electrochemical biolithography and electrostatic assembly
AU - Kaji, Hirokazu
AU - Sekine, Soichiro
AU - Abe, Takashi
AU - Nishizawa, Matsuhiko
PY - 2007/12/1
Y1 - 2007/12/1
N2 - We report a method for producing patterned cell co-cultures inside silicone tubing. A platinum needle microelectrode was inserted through the wall of the tubing and an oxidizing agent electrochemically generated at the inserted electrode. This agent caused local detachment of the anti-biofouling heparin layer from the inner surface of the tubing. The cell-adhesive protein fibronectin selectively adsorbed onto the newly exposed surface, making it possible to initiate a localized cell culture. The electrode could be readily set in place without breaking the tubular structure and, importantly, almost no culture solution leaked from the electrode insertion site after the electrode was removed. Ionic adsorption of poly-L-lysine at the tubular region retaining a heparin coating was used to switch the heparin surface from cell-repellent to cell-adhesive, thereby facilitating the adhesion of a second cell type. The combination of the electrode-based technique with electrostatic deposition enabled the formation of patterned co-cultures within the semi-closed tubular structure. The controlled co-cultures inside the elastic tubing should be of value for cell-cell interaction studies following application of chemical or mechanical stimuli and for tissue engineering-based bioreactors.
AB - We report a method for producing patterned cell co-cultures inside silicone tubing. A platinum needle microelectrode was inserted through the wall of the tubing and an oxidizing agent electrochemically generated at the inserted electrode. This agent caused local detachment of the anti-biofouling heparin layer from the inner surface of the tubing. The cell-adhesive protein fibronectin selectively adsorbed onto the newly exposed surface, making it possible to initiate a localized cell culture. The electrode could be readily set in place without breaking the tubular structure and, importantly, almost no culture solution leaked from the electrode insertion site after the electrode was removed. Ionic adsorption of poly-L-lysine at the tubular region retaining a heparin coating was used to switch the heparin surface from cell-repellent to cell-adhesive, thereby facilitating the adhesion of a second cell type. The combination of the electrode-based technique with electrostatic deposition enabled the formation of patterned co-cultures within the semi-closed tubular structure. The controlled co-cultures inside the elastic tubing should be of value for cell-cell interaction studies following application of chemical or mechanical stimuli and for tissue engineering-based bioreactors.
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U2 - 10.1109/MHS.2007.4420850
DO - 10.1109/MHS.2007.4420850
M3 - Conference contribution
AN - SCOPUS:50149117958
SN - 9781424418589
T3 - 2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS
SP - 187
EP - 192
BT - 2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS
T2 - 2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS
Y2 - 11 November 2007 through 14 November 2007
ER -