Generation of patterned cell co-cultures inside tubular structure using electrochemical biolithography and electrostatic assembly

Hirokazu Kaji, Soichiro Sekine, Takashi Abe, Matsuhiko Nishizawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We report a method for producing patterned cell co-cultures inside silicone tubing. A platinum needle microelectrode was inserted through the wall of the tubing and an oxidizing agent electrochemically generated at the inserted electrode. This agent caused local detachment of the anti-biofouling heparin layer from the inner surface of the tubing. The cell-adhesive protein fibronectin selectively adsorbed onto the newly exposed surface, making it possible to initiate a localized cell culture. The electrode could be readily set in place without breaking the tubular structure and, importantly, almost no culture solution leaked from the electrode insertion site after the electrode was removed. Ionic adsorption of poly-L-lysine at the tubular region retaining a heparin coating was used to switch the heparin surface from cell-repellent to cell-adhesive, thereby facilitating the adhesion of a second cell type. The combination of the electrode-based technique with electrostatic deposition enabled the formation of patterned co-cultures within the semi-closed tubular structure. The controlled co-cultures inside the elastic tubing should be of value for cell-cell interaction studies following application of chemical or mechanical stimuli and for tissue engineering-based bioreactors.

Original languageEnglish
Title of host publication2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS
Pages187-192
Number of pages6
DOIs
Publication statusPublished - 2007 Dec 1
Event2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS - Nagoya, Japan
Duration: 2007 Nov 112007 Nov 14

Publication series

Name2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS

Other

Other2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS
CountryJapan
CityNagoya
Period07/11/1107/11/14

ASJC Scopus subject areas

  • Artificial Intelligence
  • Computer Networks and Communications
  • Human-Computer Interaction
  • Electrical and Electronic Engineering

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