Generation of adeno-associated virus vector enabling functional expression of oxytocin receptor and fluorescence marker genes using the human eIF4G internal ribosome entry site element

Keisuke Sato, Shiori Date, Yumi Aoyagi, Yoshiyuki Kasahara, Akihiko Nawa, Hiroaki Mizukami, Shizu Hidema, Keiya Ozawa, Katsuhiko Nishimori

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We developed the AAV-Oxtr-IRES-Venus vector to rescue the oxytocin receptor (Oxtr) gene functionally at restricted regions in the brains of Oxtr knockout mice. First we chose human eIF4G gene-derived IRES to co- express Venus, a fluorescent marker gene, with Oxtr. With selected human eIF4G IRES, we constructed the AAV-Oxtr-IRES-Venus vector, and it caused expression of the Venus gene in the brain when 1 μ,l of viral solution (9.4 x 107 vg) was injected into the medial amygdaloid nucleus. In primary neuronal cells transduced with this viral vector and followed by oxytocin administration, functional expression of OXTR was detected by Ca2+ imaging assay.

Original languageEnglish
Pages (from-to)2145-2148
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume73
Issue number9
DOIs
Publication statusPublished - 2009

Keywords

  • Adeno-associated virus
  • EIF4G internal ribosome entry site
  • Oxytocin receptor

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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