Generation of a syngeneic mouse model to study the intraperitoneal dissemination of ovarian cancer with in vivo luciferase imaging

Masafumi Toyoshimaa, Yoshinori Tanaka, Mitsuyo Matumoto, Miyuki Yamazaki, Satoru Nagase, Kazuo Sugamura, Nobuo Yaegashi

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

In order to facilitate the discovery and investigation of anti-cancer therapeutics under physiological conditions, we have engineered the ovarian cancer cell line, HM-1/luc, in mice. This cell stably expresses firefly luciferase and produces light that can be detected using an in vivo imaging system (IVIS). Parental HM-1 cells cause severe carcinomatous peritonitis to B6C3F1 mice, but not to C57BL6 mice. Established HM-1/luc cells showed pathologically similar findings to HM-1 cells. HM-1/luc cells were injected into the peritoneal cavity of B6C3F1 mice and IVIS 2000 was conducted weekly after inoculation to monitor intra- peritoneal tumor growth. The mice were divided into three groups: non-CDDP-treated (control) and CDDP-treated (0.2 and 0.4 mg). A disease-suppressive effect of the CDDP was reflected by the significantly prolonged survival of the CDDP-treated mice (control 23 ± 1.9 days, CDDP 0.2 mg 29.6 ± 2.9 days; p < 0.05); the total photon and area of flux were decreased. The optical imaging of intraperitoneal tumors via in vivo bioluminescence is effective for noninvasive monitoring and semi-quantitative analysis. Our syngeneic mouse model has the relevant clinical features of ovarian cancer, which makes it a useful model for developing new ovarian cancer therapies.

Original languageEnglish
Pages (from-to)324-331
Number of pages8
JournalLuminescence
Volume24
Issue number5
DOIs
Publication statusPublished - 2009 Sep

Keywords

  • HM-1
  • In vivo imaging
  • Intraperitoneal dissemination
  • Ovarian cancer
  • Syngeneic mouse model

ASJC Scopus subject areas

  • Biophysics
  • Chemistry (miscellaneous)

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