Generation of a mouse with conditionally activated signaling through the BMP receptor, ALK2

Tomokazu Fukuda, Gregory Scott, Yoshihiro Komatsu, Runa Araya, Masako Kawano, Manas K. Ray, Masahisa Yamada, Yuji Mishina

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

BMP signaling plays pleiotropic roles in various tissues. Transgenic mouse lines that overexpress BMP signaling in a tissue-specific manner would be beneficial; however, production of each tissue-specific transgenic mouse line is labor-intensive. Here, using a Cre-loxP system, we generated a conditionally overexpressing mouse line for BMP signaling through the type I receptor ALK2 (alternatively known as AVCRI, ActRI, or ActRIA). By mating this line with Cre-expression mouse lines, Cre-mediated recombination removes an intervening floxed lacZ expression cassette and thereby permits the expression of a constitutively active form of Alk2 (caAlk2) driven by a ubiquitous promoter, CAG. Tissue specificity of Cre recombination was monitored by a bicistronically expressed EGFP following Alk2 cDNA. Increased BMP signaling was confirmed by ectopic phosphorylation of SMAD1/5/8 in the areas where Cre recombination had occurred. The conditional overexpression system described here provides versatility in investigating gene functions in a tissue-specific manner without having to generate independent tissue-specific transgenic lines.

Original languageEnglish
Pages (from-to)159-167
Number of pages9
JournalGenesis
Volume44
Issue number4
DOIs
Publication statusPublished - 2006 Apr

Keywords

  • Bone morphogenetic protein
  • Constitutively active
  • Cre-loxP system
  • Smad
  • Transgenic mouse

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology

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    Fukuda, T., Scott, G., Komatsu, Y., Araya, R., Kawano, M., Ray, M. K., Yamada, M., & Mishina, Y. (2006). Generation of a mouse with conditionally activated signaling through the BMP receptor, ALK2. Genesis, 44(4), 159-167. https://doi.org/10.1002/dvg.20201