Generation of a monoclonal antibody specific for tissue-nonspecific alkaline phosphatase and its use in a clinical diagnostic study

Tatsuya Ohashi, Masahiko Sunaga, Toshihide Miura, Kumiko Sasagawa, Yasuhito Sato, Wataru Ohashi, Ken Katagiri, Yoshinori Katano, Iwao Kiyokawa, Ryo Kojima, Takeshi Tomonaga, Fumio Nomura, Norio Amizuka, Kimimitsu Oda, Toyoji Sato, Katsuhiro Katayama

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Tissue-nonspecific alkaline phosphatase (TNSALP) in serum comprises liver alkaline phosphatase (liver-ALP) and bone alkaline phosphatase (bone-ALP). Liver-ALP is a marker of liver disease; thus a specific method for its measurement would be useful. Measurement of ALP by electrophoresis is difficult, although all of the isozymes can be assessed simultaneously. Total ALP can also be measured by automated analyzer, but it is difficult to determine the cause of a high ALP value because bone-, intestine-, placenta-, and tumor-ALP are measured together. Thus, anti-TNSALP monoclonal antibodies that can resolve these problems are needed. Here we have generated an anti-TNSALP monoclonal antibody, 3-29-3R. This clone has specificity to liver-ALP rather than to bone-ALP. In electrophoresis, 3-29-3R reacted with TNSALP and shifted the bands. The use of 3-29-3R enabled easy interpretation of the results. Furthermore, we tested 3-29-3R by developing an immunocapture enzymatic assay (IEA). Preliminary results of the IEA show that this method is effective for measurement of liver-ALP. Thus, the monoclonal antibody that we have established may be a useful tool for clinical diagnosis.

Original languageEnglish
Pages (from-to)401-406
Number of pages6
JournalHybridoma
Volume26
Issue number6
DOIs
Publication statusPublished - 2007 Dec 1
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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