Abstract
For comprehensive analysis of genes expressed in a model legume, Lotus japonicus, a total of 22,983 5′ end expressed sequence tags (ESTs) were accumulated from normalized and size-selected cDNA libraries constructed from young (2 weeks old) plants. The EST sequences were clustered into 7137 non-redundant groups. Similarity search against public non-redundant protein database indicated that 3302 groups showed similarity to genes of known function, 1143 groups to hypothetical genes, and 2692 were novel sequences. Homologues of 5 nodule-specific genes which have been reported in other legume species were contained in the collected ESTs, suggesting that the EST source generated in this study will become a useful tool for identification of genes related to legume-specific biological processes.
Original language | English |
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Pages (from-to) | 127-130 |
Number of pages | 4 |
Journal | DNA Research |
Volume | 7 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2000 |
Externally published | Yes |
Keywords
- EST
- Legume
- Lotus japonicus
- cDNA
ASJC Scopus subject areas
- Molecular Biology
- Genetics