General description: Future prospects of optogenetics

Hiromu Yawo, Ryo Egawa, Shoko Hososhima, Lei Wen

    Research output: Chapter in Book/Report/Conference proceedingChapter

    2 Citations (Scopus)

    Abstract

    Recent optical neuro-research methods have several advantages over conventional techniques: high resolution in space and time, together with direct measurement and manipulation of cell functions by light. The use of fluorescent proteins, bioluminescence systems, and light-sensitive proteins facilitates the optical methods in combination with genetic engineering techniques. Techniques involving the application of light-sensitive proteins are collectively termed ‘optogenetics’ because they combine optics and genetics. Light-sensitive proteins, either natural or synthetic, are termed ‘optogenetic molecular reagents’ (OMRs) or ‘optogenetic molecular tools’ if they are used as tools for optogenetics. These techniques are receiving recognition as enabling breakthroughs in neuroscience research. In this chapter, the topics and the prospects of optogenetics are discussed from three view points: the optimization of OMRs, the optimization of gene-targeting methods, and the optimization of optics.

    Original languageEnglish
    Title of host publicationOptogenetics
    Subtitle of host publicationLight-Sensing Proteins and their Applications
    PublisherSpringer Japan
    Pages110-132
    Number of pages23
    ISBN (Electronic)9784431555162
    ISBN (Print)9784431555155
    DOIs
    Publication statusPublished - 2015 Jan 1

    Keywords

    • Channelrhodopsin
    • Electroporation
    • Flavoprotein
    • Optogenetics
    • Phytochrome
    • Transgenic
    • Transporter-type rhodopsin
    • Two-Photon microscopy
    • Viral vector

    ASJC Scopus subject areas

    • Medicine(all)
    • Neuroscience(all)
    • Biochemistry, Genetics and Molecular Biology(all)

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