Gene trapping in Arabidopsis reveals genes involved in vascular development

Shingo Nagawa, Shinichirou Sawa, Shusei Sato, Tomohiko Kato, Satoshi Tabata, Hiroo Fukuda

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The procambium is made up of stem cells that give rise to various vascular cells in plants. To understand the molecular nature of procambium cells, we tried to identify genes that characterize procambium cells using Arabidopsis gene trap lines. Among 26,000 gene trap lines, we found 67 lines in which β-glucuronidase (GUS) staining occurred along vascular tissues in cotyledons and/or adult leaves. Although four gene trap lines showed procambium-preferential GUS expression, their expression patterns differed from each other during procambium development in root tips and young rosette leaves. Genomic regions flanking the gene trap insertion points in 25 of the 67 lines were determined, including three lines showing preferential GUS staining of the procambium. The three procambium-related genes encoded PINHEAD, katanin and an unknown DUF740 domain-containing protein. We discuss procambium development based on the functions and the differential GUS staining patterns of the procambium-related genes.

Original languageEnglish
Pages (from-to)1394-1405
Number of pages12
JournalPlant and Cell Physiology
Volume47
Issue number10
DOIs
Publication statusPublished - 2006 Oct 1
Externally publishedYes

Keywords

  • Arabidopsis thaliana
  • Gene expression pattern
  • Gene trap lines
  • Procambium
  • Vascular development
  • β-Glucuronidase

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

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