Gene transfer into cultured mammalian embryos by electroporation

Masanori Takahashi, Takako Kikkawa, Noriko Osumi

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

Electroporation has been widely used in various animals to introduce transgenes into their tissues and organs. We have previously developed a gene transfer method into the developing brain of rat and mouse embryos by applying electroporation to a mammalian whole embryo culture technique. We can directly transfer exogenous genes and small nucleic acids, e.g., double strand RNAs, siRNAs, and Morpholino oligos, into desirable regions of the developing brain of cultured embryos at different stages by easily adjusting the direction of electrodes. This method enables us to provide simple and convenient gain-of- function and loss-of-function studies to explore novel understanding of molecular and cellular mechanisms underlying mammalian brain development.

Original languageEnglish
Title of host publicationElectroporation Methods in Neuroscience
PublisherSpringer New York
Pages141-157
Number of pages17
ISBN (Electronic)9781493924592
ISBN (Print)9781493924585
DOIs
Publication statusPublished - 2015 Mar 9

Keywords

  • Brain
  • Electroporation
  • Mammalian whole embryo culture
  • Mouse
  • Rat

ASJC Scopus subject areas

  • Medicine(all)
  • Neuroscience(all)

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    Takahashi, M., Kikkawa, T., & Osumi, N. (2015). Gene transfer into cultured mammalian embryos by electroporation. In Electroporation Methods in Neuroscience (pp. 141-157). Springer New York. https://doi.org/10.1007/978-1-4939-2459-2_11