Gene transduction by sonoporation

Sho Ohta; Kentaro Suzuki; Yukiko Ogino; Shinichi Miyagawa; Aki Murashima; Daisuke Matsumaru; Gen Yamada

doi:10.1111/j.1440-169X.2008.01026.x

Gene transduction by sonoporation

Sho Ohta, Kentaro Suzuki, Yukiko Ogino, Shinichi Miyagawa, Aki Murashima, Daisuke Matsumaru, Gen Yamada

Research output: Contribution to journal › Short survey › peer-review

30 Citations (Scopus)

Abstract

Gene transduction technologies are essential tools for understanding of gene functions or gene cascades underlying embryogenesis. In this review, we introduce a gene transduction method using microbubble and ultrasound (hereafter referred to as sonoporation). Sonoporation is carried out with relatively simple procedures and easily transduces genes into mesenchymal cells without significant damage to target tissues. Therefore, sonoporation is effective for gene transduction to study the molecular mechanisms of morphogenesis.

Original language	English
Pages (from-to)	517-520
Number of pages	4
Journal	Development Growth and Differentiation
Volume	50
Issue number	6
DOIs	https://doi.org/10.1111/j.1440-169X.2008.01026.x
Publication status	Published - 2008 Aug 1
Externally published	Yes

Keywords

Gene transduction chick
Limb bud
Microbubble
Sonoporation
Ultrasound

ASJC Scopus subject areas

Developmental Biology
Cell Biology

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AU - Ohta, Sho

AU - Suzuki, Kentaro

AU - Ogino, Yukiko

AU - Miyagawa, Shinichi

AU - Murashima, Aki

AU - Matsumaru, Daisuke

AU - Yamada, Gen

PY - 2008/8/1

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N2 - Gene transduction technologies are essential tools for understanding of gene functions or gene cascades underlying embryogenesis. In this review, we introduce a gene transduction method using microbubble and ultrasound (hereafter referred to as sonoporation). Sonoporation is carried out with relatively simple procedures and easily transduces genes into mesenchymal cells without significant damage to target tissues. Therefore, sonoporation is effective for gene transduction to study the molecular mechanisms of morphogenesis.

AB - Gene transduction technologies are essential tools for understanding of gene functions or gene cascades underlying embryogenesis. In this review, we introduce a gene transduction method using microbubble and ultrasound (hereafter referred to as sonoporation). Sonoporation is carried out with relatively simple procedures and easily transduces genes into mesenchymal cells without significant damage to target tissues. Therefore, sonoporation is effective for gene transduction to study the molecular mechanisms of morphogenesis.

KW - Gene transduction chick

KW - Limb bud

KW - Microbubble

KW - Sonoporation

KW - Ultrasound

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