Gene-specific requirement of a nuclear protein, IκB-ζ, for promoter association of inflammatory transcription regulators

Soh Yamazaki, Susumu Matsuo, Tatsushi Muta, Masahiro Yamamoto, Shizuo Akira, Koichiro Takeshige

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)


Expression of many inflammatory genes is induced through activation of the transcription factor NF-κB. In contrast to the advanced understanding of cytoplasmic control of NF-κB activation, its regulation in the nucleus has not been fully understood despite its importance in selective gene expression. We previously identified an inducible nuclear protein, IκB-ζ, and demonstrated that this molecule is indispensable for the expression of a group of NF-κB-regulated genes. In this study, we established a unique gene induction system, in which IκB-ζ is expressed independently of inflammatory stimuli, to specifically investigate the molecular basis underlying IκB-ζ-mediated gene activation. We show that in the presence of IκB-ζ other primary response genes are dispensable for the expression of the target secondary response genes. ChIP analyses revealed that IκB-ζ is required for stimulus-induced recruitment of NF-κB onto the target promoter in a gene-specific manner. Surprisingly, IκB-ζ is also necessary for the gene-selective promoter recruitment of another inflammatory transcription factor, C/EBPβ, and the chromatin remodeling factor Brg1. We propose a new gene regulatory mechanism underlying the selective expression of inflammatory genes.

Original languageEnglish
Pages (from-to)32404-32411
Number of pages8
JournalJournal of Biological Chemistry
Issue number47
Publication statusPublished - 2008 Nov 21

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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