Gene regulatory network and its constituent transcription factors that control nitrogen-deficiency responses in rice

Yoshiaki Ueda, Namie Ohtsuki, Koji Kadota, Ayumi Tezuka, Atsushi J. Nagano, Taro Kadowaki, Yonghyun Kim, Mitsue Miyao, Shuichi Yanagisawa

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Increase in the nitrogen (N)-use efficiency and optimization of N response in crop species are urgently needed. Although transcription factor-based genetic engineering is a promising approach for achieving these goals, transcription factors that play key roles in the response to N deficiency have not been studied extensively. Here, we performed RNA-seq analysis of root samples of 20 Asian rice (Oryza sativa) accessions with differential nutrient uptake. Data obtained from plants exposed to N-replete and N-deficient conditions were subjected to coexpression analysis and machine learning-based pathway inference to dissect the gene regulatory network required for the response to N deficiency. Four transcription factors, including members of the G2-like and bZIP families, were predicted to function as key regulators of gene transcription within the network in response to N deficiency. Cotransfection assays validated inferred novel regulatory pathways, and further analyses using genome-edited knockout lines suggested that these transcription factors are important for N-deficiency responses in planta. Many of the N deficiency-responsive genes, including those encoding key regulators within the network, were coordinately regulated by transcription factors belonging to different families. Transcription factors identified in this study could be valuable for the modification of N response and metabolism.

Original languageEnglish
Pages (from-to)1434-1452
Number of pages19
JournalNew Phytologist
Volume227
Issue number5
DOIs
Publication statusPublished - 2020 Sep 1

Keywords

  • coexpression
  • gene regulatory network
  • genome editing
  • machine learning
  • nitrogen
  • rice
  • systems biology
  • transcription factor

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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  • Cite this

    Ueda, Y., Ohtsuki, N., Kadota, K., Tezuka, A., Nagano, A. J., Kadowaki, T., Kim, Y., Miyao, M., & Yanagisawa, S. (2020). Gene regulatory network and its constituent transcription factors that control nitrogen-deficiency responses in rice. New Phytologist, 227(5), 1434-1452. https://doi.org/10.1111/nph.16627