Gene regulation of a novel angiogenesis inhibitor, vasohibin, in endothelial cells

Kazue Shimizu, Kazuhide Watanabe, Hiroshi Yamashita, Mayumi Abe, Hironobu Yoshimatsu, Hideki Ohta, Hikaru Sonoda, Yasufumi Sato

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

We recently reported that vasohibin is a negative feedback regulator of angiogenesis, and it is specifically expressed in endothelial cells. Here, we characterize the regulation of vasohibin expression. Two possible splicing variants were found, and the longer isoform was preferentially expressed. VEGF induced the expression of vasohibin, and this induction was abrogated by anti-VEGFR2 mAb but not by anti-VEGFR1 mAb. Pharmacological analysis revealed that the downstream targets of VEGFR2 were PKCs, especially PKCδ. Actinomycin D did not alter the kinetics of vasohibin mRNA induction upon VEGF treatment, whereas cycloheximide completely abolished its induction. We tested the effect of various inflammatory cytokines on vasohibin expression. TNFα, IL1 and IFNγ decreased VEGF-stimulated vasohibin expression. Actinomycin D did not alter the kinetics of vasohibin mRNA induction upon TNFα treatment. These results indicate that the expression of vasohibin in endothelial cells is regulated either positively or negatively by certain factors at the transcriptional level.

Original languageEnglish
Pages (from-to)700-706
Number of pages7
JournalBiochemical and biophysical research communications
Volume327
Issue number3
DOIs
Publication statusPublished - 2005 Feb 18

Keywords

  • Angiogenesis inhibitor
  • Endothelial cell
  • Induction
  • PKC
  • VEGF
  • Vasohibin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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