Gene rearrangements of T cell receptor β and γ chains in HTLV-I infected primary neoplastic T cells

M. Matsuoka, M. Hagiya, T. Hattori, N. Asou, S. Maeda, K. Shimada, S. C. Tsai, H. Sakano, K. Takatsuki

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19 Citations (Scopus)

Abstract

Rearrangements of T cell receptor β and γ chain (Tβ and Tγ) genes were analyzed by Southern blot method in samples from 30 patients with adult T cell leukemia (ATL) and 17 patients with non-ATL T cell neoplasms. The DNA probes used were the constant and joining region of Tβ and the joining region of Tγ gene. Rearranged bands of Tβ gene on one or both allelic chromosomes were detected in all neoplastic T cells, even those of smoldering ATL, in which only a small percentage of peripheral blood T cells were detected as leukemic. Tγ gene was rearranged in the cells of all but one patient, the exception being one ATL patient. In order to test whether any given variable region (V) of Tβ gene was expressed in ATL cells, two functionally rearranged Vβ sequences of ATL were compared with a Vβ sequence from T cells acute lymphoblastic leukemia cells. No significant homologies were noted among the three deduced gene product amino acid sequences, confirming that Tβ molecules of ATL cells contained no specific structures in common. The observed heterogeneity of Tβ and Tγ gene rearrangements in ATL cells further supported these findings.

Original languageEnglish
Pages (from-to)84-90
Number of pages7
JournalLeukemia
Volume2
Issue number2
Publication statusPublished - 1988 Jan 1

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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    Matsuoka, M., Hagiya, M., Hattori, T., Asou, N., Maeda, S., Shimada, K., Tsai, S. C., Sakano, H., & Takatsuki, K. (1988). Gene rearrangements of T cell receptor β and γ chains in HTLV-I infected primary neoplastic T cells. Leukemia, 2(2), 84-90.