Gene manipulation of chick embryos in vitro, early chick culture, and long survival in transplanted eggs

Jun Tanaka, Hidekiyo Harada, Kodai Ito, Toshihiko Ogura, Harukazu Nakamura

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

We introduce a revolutionary gene transfer system in chick: transfect chick embryos at early developmental stage by electroporation in vitro, Early Chick (EC) culture, and transplant to the egg to let the embryo survive until E5.5. Referring to the fate map, we could target the tissues of transfection, or transfect large areas of the embryo. We could get tissue-specific expression of a transgene by tissue-specific promoter. This method is very convenient and rapid, but allows us to get stable expression of the transgene in combination with transposon system.

Original languageEnglish
Pages (from-to)629-634
Number of pages6
JournalDevelopment Growth and Differentiation
Volume52
Issue number7
DOIs
Publication statusPublished - 2010 Sep

Keywords

  • Early Chick culture
  • chick
  • electroporation
  • heart
  • transplantation

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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