Gene manipulation of chick embryos in vitro, early chick culture, and long survival in transplanted eggs

Jun Tanaka; Hidekiyo Harada; Kodai Ito; Toshihiko Ogura; Harukazu Nakamura

doi:10.1111/j.1440-169X.2010.01198.x

Gene manipulation of chick embryos in vitro, early chick culture, and long survival in transplanted eggs

Jun Tanaka, Hidekiyo Harada, Kodai Ito, Toshihiko Ogura, Harukazu Nakamura

Division of Brain Science

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

We introduce a revolutionary gene transfer system in chick: transfect chick embryos at early developmental stage by electroporation in vitro, Early Chick (EC) culture, and transplant to the egg to let the embryo survive until E5.5. Referring to the fate map, we could target the tissues of transfection, or transfect large areas of the embryo. We could get tissue-specific expression of a transgene by tissue-specific promoter. This method is very convenient and rapid, but allows us to get stable expression of the transgene in combination with transposon system.

Original language	English
Pages (from-to)	629-634
Number of pages	6
Journal	Development Growth and Differentiation
Volume	52
Issue number	7
DOIs	https://doi.org/10.1111/j.1440-169X.2010.01198.x
Publication status	Published - 2010 Sep

Keywords

Early Chick culture
chick
electroporation
heart
transplantation

ASJC Scopus subject areas

Developmental Biology
Cell Biology

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AU - Tanaka, Jun

AU - Harada, Hidekiyo

AU - Ito, Kodai

AU - Ogura, Toshihiko

AU - Nakamura, Harukazu

PY - 2010/9

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AB - We introduce a revolutionary gene transfer system in chick: transfect chick embryos at early developmental stage by electroporation in vitro, Early Chick (EC) culture, and transplant to the egg to let the embryo survive until E5.5. Referring to the fate map, we could target the tissues of transfection, or transfect large areas of the embryo. We could get tissue-specific expression of a transgene by tissue-specific promoter. This method is very convenient and rapid, but allows us to get stable expression of the transgene in combination with transposon system.

KW - Early Chick culture

KW - chick

KW - electroporation

KW - heart

KW - transplantation

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