Gene expression profiles of cryopreserved CD34+ human umbilical cord blood cells are related to their bone marrow reconstitution abilities in mouse xenografts

Kazuhiro Sudo, Jun Yasuda, Yukio Nakamura

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Human umbilical cord blood (UCB) cells are an alternative source of hematopoietic stem cells for treatment of leukemia and other diseases. It is very difficult to assess the quality of UCB cells in the clinical situation. Here, we sought to assess the quality of UCB cells by transplantation to immunodeficient mice. Cryopreserved CD34+ UCB cells from twelve different human donors were transplanted into sublethally irradiated NOD/shi-scid Jic mice. In parallel, the gene expression profiles of the UCB cells were determined from oligonucleotide microarrays. UCB cells from three donors failed to establish an engraftment in the host mice, while the other nine succeeded to various extents. Gene expression profiling indicated that 71 genes, including HOXB4, C/EBP-β, and ETS2, were specifically overexpressed and 23 genes were suppressed more than 2-fold in the successful UCB cells compared to those that failed. Functional annotation revealed that cell growth and cell cycle regulators were more abundant in the successful UCB cells. Our results suggest that hematopoietic ability may vary among cryopreserved UCB cells and that this ability can be distinguished by profiling expression of certain sets of genes.

Original languageEnglish
Pages (from-to)697-705
Number of pages9
JournalBiochemical and biophysical research communications
Volume397
Issue number4
DOIs
Publication statusPublished - 2010 Jul

Keywords

  • Gene expression profile
  • Transplantation
  • Umbilical cord blood

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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