Gene expression profiles in mouse liver cells after exposure to different types of radiation

Mehryar Habibi Roudkenar, Li Li, Taisuke Baba, Yoshikazu Kuwahara, Hironobu Nakagawa, Lu Wang, Satoshi Kasaoka, Yasuhito Ohkubo, Koji Ono, Manabu Fukumoto

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

The liver is one of the target organs of radiation-induced cancers by internal exposures. In order to elucidate radiation-induced liver cancers including Thorotrast, we present a new approach to investigate in vivo effects of internal exposure to α-particles. Adopting boron neutron capture, we separately irradiated Kupffer cells and endothelial cells in mouse liver in vivo and analyzed the changes in gene transcriptions by an oligonucleotide microarray. Differential expression was defined as more than 3-fold for upregulation and less than 1/3 for under-regulation, compared with non-irradiated controls. Of 6,050 genes examined, 68 showed differential expression compared with non-irradiated mice. Real-time polymerase chain reaction validated the results of the microarray analysis. Exposure to α-particles and γ-rays produced different patterns of altered gene expression. Gene expression profiles revealed that the liver was in an inflammatory state characterized by up-regulation of positive acute phase protein genes, irrespective of the target cells exposed to radiation. In comparison with chemical and biological hepatotoxicants, inductions of Metallothionein 1 and Hemopexin, and suppressions of cytochrome P450s are characteristic of radiation exposure. Anti-inflammatory treatment could be helpful for the prevention and protection of radiationinduced hepatic injury.

Original languageEnglish
Pages (from-to)29-40
Number of pages12
JournalJournal of radiation research
Volume49
Issue number1
DOIs
Publication statusPublished - 2008
Externally publishedYes

Keywords

  • Endothelial cell
  • Gene expression
  • Kupffer cell
  • Microarray
  • Mouse liver
  • Radiation quality

ASJC Scopus subject areas

  • Radiation
  • Radiology Nuclear Medicine and imaging
  • Health, Toxicology and Mutagenesis

Fingerprint Dive into the research topics of 'Gene expression profiles in mouse liver cells after exposure to different types of radiation'. Together they form a unique fingerprint.

Cite this