Gene expression of (pro)renin receptor is upregulated in hearts and kidneys of rats with congestive heart failure

Takuo Hirose, Nobuyoshi Mori, Kazuhito Totsune, Ryo Morimoto, Takahiro Maejima, Takuya Kawamura, Hirohito Metoki, Kei Asayama, Masahiro Kikuya, Takayoshi Ohkubo, Masahiro Kohzuki, Kazuhiro Takahashi, Yutaka Imai

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Recent studies have revealed that (pro)renin receptor ((P)RR), a newly identified member of the renin-angiotensin system, was associated with organ damage in the kidney. However, there has been little information for (P)RR in hearts. To investigate the regulation of (P)RR in heart failure, we examined the expression of (P)RR in hearts and kidneys of rats with congestive heart failure (CHF) due to coronary ligation by quantitative RT-PCR and immunohistochemistry. Significantly increased levels of (P)RR mRNA were found in the atrium, right ventricle, non-infarcted part of left ventricle, infarcted part of left ventricle and kidney of CHF rats, when compared with sham operated rats (about 1.6-fold, 1.4-fold, 1.6-fold, 1.7-fold and 1.5-fold, respectively). Expression levels of mRNAs encoding renin and angiotensinogen in these heart and kidney tissues were also increased in the CHF rats. Immunohistochemistry showed positive (P)RR immunostaining in the myocardium, the renal tubular cells, and vascular smooth muscle and endothelial cells in the heart and the kidney. The renal tubular cells were more intensely immunostained in CHF rats than in sham operated rats. These findings suggest that the expression of (P)RR is increased in the hearts and kidneys of rats with heart failure, and that (P)RR may contribute to heart failure.

Original languageEnglish
Pages (from-to)2316-2322
Number of pages7
JournalPeptides
Volume30
Issue number12
DOIs
Publication statusPublished - 2009 Dec

Keywords

  • Immunohistochemistry
  • Organ damage
  • RT-PCR
  • Renin-angiotensin system

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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