TY - JOUR
T1 - Gene expression of fibroblast growth factors in human gliomas and meningiomas
T2 - Demonstration of cellular source of basic fibroblast growth factor mRNA and peptide in tumor tissues
AU - Takahashi, Jun A.
AU - Mori, Hirotaka
AU - Fukumoto, Manabu
AU - Igarashi, Koichi
AU - Jaye, Michael
AU - Oda, Yoshifumi
AU - Kikuchi, Haruhiko
AU - Hatanaka, Masakazu
PY - 1990
Y1 - 1990
N2 - The growth autonomy of human tumor cells is considered due to the endogenous production of growth factors. Transcriptional expression of candidates for autocrine stimulatory factors such as basic fibroblast growth factor (FGF), acidic FGF, and transforming growth factor type were determined in human brain tumors. Basic FGF was expressed abundantly in 17 of 18 gliomas, 20 of 22 meningiomas, and 0 of 5 metastatic brain tumors. The level of mRNA expression of acidic FGF in gliomas was significant. In contrast, transforming growth factor type β1 was expressed in all the samples investigated. The mRNA for basic FGF and its peptide were localized in tumor cells in vivo by in situ hybridization and immunohistochemistry, showing that basic FGF is actually produced in tumor cells. Our results suggest that tumor-derived basic FGF is involved in the progression of gliomas and meningiomas in vivo, whereas acidic FGF is expressed in a tumor origin-specific manner, suggesting that acidic FGF works in tandem with basic FGF in glioma tumorigenesis.
AB - The growth autonomy of human tumor cells is considered due to the endogenous production of growth factors. Transcriptional expression of candidates for autocrine stimulatory factors such as basic fibroblast growth factor (FGF), acidic FGF, and transforming growth factor type were determined in human brain tumors. Basic FGF was expressed abundantly in 17 of 18 gliomas, 20 of 22 meningiomas, and 0 of 5 metastatic brain tumors. The level of mRNA expression of acidic FGF in gliomas was significant. In contrast, transforming growth factor type β1 was expressed in all the samples investigated. The mRNA for basic FGF and its peptide were localized in tumor cells in vivo by in situ hybridization and immunohistochemistry, showing that basic FGF is actually produced in tumor cells. Our results suggest that tumor-derived basic FGF is involved in the progression of gliomas and meningiomas in vivo, whereas acidic FGF is expressed in a tumor origin-specific manner, suggesting that acidic FGF works in tandem with basic FGF in glioma tumorigenesis.
KW - Acidic fibroblast growth factor
KW - Brain neoplasm
KW - In situ hybridization
KW - Northern blot analysis
KW - Transforming growth factor type β1
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U2 - 10.1073/pnas.87.15.5710
DO - 10.1073/pnas.87.15.5710
M3 - Article
C2 - 2377607
AN - SCOPUS:0025292855
VL - 87
SP - 5710
EP - 5714
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 15
ER -