Gene expression analysis of CCN protein in bone under mechanical stress

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

To investigate mechanical-dependent bone remodeling, we had previously applied various types of mechanical loading onto the teeth of rats and mice. In vitro cultured bone cells were then used to elucidate the mechanisms underlying the specifi c phenomenon revealed by in vivo experiments. This review describes the techniques used to upregulate CCN2 expression in bone cells produced by different types of mechanical stress, such as fl uid shear stress and substrate strain in vitro, and compression or tension force in vivo.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages283-308
Number of pages26
DOIs
Publication statusPublished - 2017 Jan 1

Publication series

NameMethods in Molecular Biology
Volume1489
ISSN (Print)1064-3745

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Gene expression analysis of CCN protein in bone under mechanical stress'. Together they form a unique fingerprint.

  • Cite this

    Yamamoto, T., Fukunaga, T., & Takeshita, N. (2017). Gene expression analysis of CCN protein in bone under mechanical stress. In Methods in Molecular Biology (pp. 283-308). (Methods in Molecular Biology; Vol. 1489). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6430-7_26