Gene expression analyses of human macrophage phagocytizing sub-μ titanium particles by allergy DNA chip (Genopal™)

Masayuki Taira, Takashi Nezu, Minoru Sasaki, Shigenobu Kimura, Tadayoshi Kagiya, Hidemitsu Harada, Takayuki Narushima, Yoshima Araki

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

The purpose of this study was to examine gene expressions of macrophage phagocytizing sub-μ Ti particles by a DNA chip. Human monocytic cell line THP-1 was differentiated into macrophages by culturing for two days in medium supplemented with 200 nM phorbol ester (PMA). The macrophages were then cultured in four media: medium without PMA (control); medium with suspended sub-μ Ti particles (0.5 wt%); medium with 1.0 μg/ml lipopolysaccharide (LPS); and medium with LPS and Ti particles. After 6 hours' culture, total RNA were extracted and gene expressions were evaluated by DNA allergy chip with 205 allergy and inflammation related gene spots. We found that phagocytosis of sub-μ Ti particles and LPS independently and synergistically up-regulated 17 inflammation-related genes more than two-fold. The extensive expressions of four genes (CCL1, IL1B, IL6 and IL8) were further confirmed by real-time quantitative PCR. It turned out that dual stimulation of LPS and Ti particles most up-regulated three genes (IL1B, IL6 and IL8), followed by LPS while Ti particles moderately but least increased, suggesting that phagocytosis of sub-μ Ti particles induces moderate inflammation with its degree less than LPS, but phagocytosis of sub-μ Ti particles has the potential to worsen inflammation caused by LPS-stimulated macrophages.

Original languageEnglish
Pages (from-to)63-70
Number of pages8
JournalBio-medical materials and engineering
Volume19
Issue number1
DOIs
Publication statusPublished - 2009 Jun 8

Keywords

  • DNA allergy chip
  • Gene expression
  • Macrophage
  • Phagocytosis
  • Sub-μ titanium particles

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

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