Gene dosage and genetic background affect miraculin accumulation in transgenic tomato fruits

You Wang Kim, Tadayoshi Hirai, Kazuhisa Kato, Kyoko Hiwasa-Tanase, Hiroshi Ezura

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Gene dosage and genetic background are factors that influence transgene expression in transgenic plants. In our previous studies, we produced transgenic tomato plants that accumulate miraculin, a taste-modifying protein, in a genetically stable manner. To elucidate the effects of gene dosage and genetic background on miraculin accumulation in transgenic tomato fruits, we generated hybrid tomato lines between the homozygous transgenic line 56B (background cultivar 'Moneymaker') and the pure cultivars 'Micro-Tom,' 'Moneymaker,' 'Ailsa Craig,' 'M82,' 'Rutgers' and 'Aichi-first' and analyzed them for miraculin mRNA expression and miraculin protein accumulation. The hybrid lines exhibited variation in their fruit structures. Among the hybrid lines heterozygous for the miraculin gene, miraculin accumulation in the fruits varied from 111.0μgg_1 fresh weight (FW) to 159.4μgg_1FW. Furthermore, the homozygous line 56B showed higher miraculin accumulation and miraculin mRNA expression than the heterozygous line 56B× 'Moneymaker.' These results demonstrate the profound effects of gene dosage and genetic background on miraculin accumulation in transgenic tomato fruits.

Original languageEnglish
Pages (from-to)333-338
Number of pages6
JournalPlant Biotechnology
Volume27
Issue number4
DOIs
Publication statusPublished - 2010
Externally publishedYes

Keywords

  • Gene dosage
  • Genetic background
  • Miraculin
  • Taste-modifying protein

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

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