TY - JOUR
T1 - Gene dosage and genetic background affect miraculin accumulation in transgenic tomato fruits
AU - Kim, You Wang
AU - Hirai, Tadayoshi
AU - Kato, Kazuhisa
AU - Hiwasa-Tanase, Kyoko
AU - Ezura, Hiroshi
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2010
Y1 - 2010
N2 - Gene dosage and genetic background are factors that influence transgene expression in transgenic plants. In our previous studies, we produced transgenic tomato plants that accumulate miraculin, a taste-modifying protein, in a genetically stable manner. To elucidate the effects of gene dosage and genetic background on miraculin accumulation in transgenic tomato fruits, we generated hybrid tomato lines between the homozygous transgenic line 56B (background cultivar 'Moneymaker') and the pure cultivars 'Micro-Tom,' 'Moneymaker,' 'Ailsa Craig,' 'M82,' 'Rutgers' and 'Aichi-first' and analyzed them for miraculin mRNA expression and miraculin protein accumulation. The hybrid lines exhibited variation in their fruit structures. Among the hybrid lines heterozygous for the miraculin gene, miraculin accumulation in the fruits varied from 111.0μgg_1 fresh weight (FW) to 159.4μgg_1FW. Furthermore, the homozygous line 56B showed higher miraculin accumulation and miraculin mRNA expression than the heterozygous line 56B× 'Moneymaker.' These results demonstrate the profound effects of gene dosage and genetic background on miraculin accumulation in transgenic tomato fruits.
AB - Gene dosage and genetic background are factors that influence transgene expression in transgenic plants. In our previous studies, we produced transgenic tomato plants that accumulate miraculin, a taste-modifying protein, in a genetically stable manner. To elucidate the effects of gene dosage and genetic background on miraculin accumulation in transgenic tomato fruits, we generated hybrid tomato lines between the homozygous transgenic line 56B (background cultivar 'Moneymaker') and the pure cultivars 'Micro-Tom,' 'Moneymaker,' 'Ailsa Craig,' 'M82,' 'Rutgers' and 'Aichi-first' and analyzed them for miraculin mRNA expression and miraculin protein accumulation. The hybrid lines exhibited variation in their fruit structures. Among the hybrid lines heterozygous for the miraculin gene, miraculin accumulation in the fruits varied from 111.0μgg_1 fresh weight (FW) to 159.4μgg_1FW. Furthermore, the homozygous line 56B showed higher miraculin accumulation and miraculin mRNA expression than the heterozygous line 56B× 'Moneymaker.' These results demonstrate the profound effects of gene dosage and genetic background on miraculin accumulation in transgenic tomato fruits.
KW - Gene dosage
KW - Genetic background
KW - Miraculin
KW - Taste-modifying protein
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U2 - 10.5511/plantbiotechnology.27.333
DO - 10.5511/plantbiotechnology.27.333
M3 - Article
AN - SCOPUS:78049491460
VL - 27
SP - 333
EP - 338
JO - Plant Biotechnology
JF - Plant Biotechnology
SN - 1342-4580
IS - 4
ER -