Gene conversion from SLG to SRK resulting in self-compatibility in Brassica rapa

Ryo Fujimoto; Tetsu Sugimura; Takeshi Nishio

doi:10.1016/j.febslet.2005.12.028

Gene conversion from SLG to SRK resulting in self-compatibility in Brassica rapa

Ryo Fujimoto, Tetsu Sugimura, Takeshi Nishio

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

Self-compatible S-54 homozygotic plants were found in progenies of an F₁ hybrid cultivar in Chinese cabbage. Pollination tests revealed that this self-compatibility is controlled by the S locus and caused by the loss of the recognition function of the stigma. SRK, the gene for the recognition molecule in the stigma, was normally transcribed and translated in the self-compatible plants. The 1034-bp region in the receptor domain of SRK in the self-compatible plants was 100% identical to SLG in S-54, while that in self-incompatible S-54 homozygotic plants was 95.1% identical. These results suggest that the self-compatibility of the S-54 homozygotes is due to amino-acid changes caused by gene conversion from SLG to SRK.

Original language	English
Pages (from-to)	425-430
Number of pages	6
Journal	FEBS Letters
Volume	580
Issue number	2
DOIs	https://doi.org/10.1016/j.febslet.2005.12.028
Publication status	Published - 2006 Jan 23

Keywords

Brassica rapa
Gene conversion
Pollen-stigma interaction
Recognition specificity
S haplotype
Self-incompatibility

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2005.12.028

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title = "Gene conversion from SLG to SRK resulting in self-compatibility in Brassica rapa",

abstract = "Self-compatible S-54 homozygotic plants were found in progenies of an F1 hybrid cultivar in Chinese cabbage. Pollination tests revealed that this self-compatibility is controlled by the S locus and caused by the loss of the recognition function of the stigma. SRK, the gene for the recognition molecule in the stigma, was normally transcribed and translated in the self-compatible plants. The 1034-bp region in the receptor domain of SRK in the self-compatible plants was 100% identical to SLG in S-54, while that in self-incompatible S-54 homozygotic plants was 95.1% identical. These results suggest that the self-compatibility of the S-54 homozygotes is due to amino-acid changes caused by gene conversion from SLG to SRK.",

keywords = "Brassica rapa, Gene conversion, Pollen-stigma interaction, Recognition specificity, S haplotype, Self-incompatibility",

author = "Ryo Fujimoto and Tetsu Sugimura and Takeshi Nishio",

note = "Funding Information: We express our sincere thanks to Prof. Hiroshi Nyunoya in Tokyo University of Agriculture and Technology for providing the anti-SLG antibody and to Dr. Yutaka Sato for his technical advice. This work was supported in part by a Grant-in-Aid for Fundamental Research (No. 16380002) from the Ministry of Education, Science, Sports and Culture, Japan.",

year = "2006",

month = jan,

day = "23",

doi = "10.1016/j.febslet.2005.12.028",

language = "English",

volume = "580",

pages = "425--430",

journal = "FEBS Letters",

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T1 - Gene conversion from SLG to SRK resulting in self-compatibility in Brassica rapa

AU - Fujimoto, Ryo

AU - Sugimura, Tetsu

AU - Nishio, Takeshi

N1 - Funding Information: We express our sincere thanks to Prof. Hiroshi Nyunoya in Tokyo University of Agriculture and Technology for providing the anti-SLG antibody and to Dr. Yutaka Sato for his technical advice. This work was supported in part by a Grant-in-Aid for Fundamental Research (No. 16380002) from the Ministry of Education, Science, Sports and Culture, Japan.

PY - 2006/1/23

Y1 - 2006/1/23

N2 - Self-compatible S-54 homozygotic plants were found in progenies of an F1 hybrid cultivar in Chinese cabbage. Pollination tests revealed that this self-compatibility is controlled by the S locus and caused by the loss of the recognition function of the stigma. SRK, the gene for the recognition molecule in the stigma, was normally transcribed and translated in the self-compatible plants. The 1034-bp region in the receptor domain of SRK in the self-compatible plants was 100% identical to SLG in S-54, while that in self-incompatible S-54 homozygotic plants was 95.1% identical. These results suggest that the self-compatibility of the S-54 homozygotes is due to amino-acid changes caused by gene conversion from SLG to SRK.

AB - Self-compatible S-54 homozygotic plants were found in progenies of an F1 hybrid cultivar in Chinese cabbage. Pollination tests revealed that this self-compatibility is controlled by the S locus and caused by the loss of the recognition function of the stigma. SRK, the gene for the recognition molecule in the stigma, was normally transcribed and translated in the self-compatible plants. The 1034-bp region in the receptor domain of SRK in the self-compatible plants was 100% identical to SLG in S-54, while that in self-incompatible S-54 homozygotic plants was 95.1% identical. These results suggest that the self-compatibility of the S-54 homozygotes is due to amino-acid changes caused by gene conversion from SLG to SRK.

KW - Brassica rapa

KW - Gene conversion

KW - Pollen-stigma interaction

KW - Recognition specificity

KW - S haplotype

KW - Self-incompatibility

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JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

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Gene conversion from SLG to SRK resulting in self-compatibility in Brassica rapa

Abstract

Keywords

ASJC Scopus subject areas

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