Gene cluster for biosynthesis of thermophilin 1277 - a lantibiotic produced by Streptococcus thermophilus SBT1277, and heterologous expression of TepI, a novel immunity peptide

T. Kabuki, Y. Kawai, H. Uenishi, Y. Seto, J. Kok, H. Nakajima, T. Saito

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Aims: To identify genes cluster for thermophilin 1277 produced by Streptococcus thermophilus SBT1277. Methods and Results: To identify genes for thermophilin 1277 production, the chromosomal DNA region surrounding the structural gene, tepA, was sequenced using a primer-walking method. The thermophilin 1277 biosynthesis gene locus (tep) is a 9·9-kb region, which consists of at least ten open reading frames (ORFs) in the following order: tepAMTFEGKRI and ORF4. Homology analysis showed high similarity to genes involved in bovicin HJ50 production by Streptococcus bovis HJ50. tepI encodes a novel, small, positively charged hydrophobic peptide of 52 amino acids, which contains a putative transmembrane segment. By heterologous expression in Lactococcus lactis ssp. cremoris MG1363, the TepI-expressing strain exhibited at least 1·3 times higher resistance to thermophilin 1277. Conclusions: Thermophilin 1277 biosynthesis genes were encoded by a 9·9-kbp region containing at least ten ORFs. TepI is a novel immunity peptide, which protected Strep. thermophilus SBT1277 against thermophilin 1277 in addition to TepFEG, a putative ABC transporter. Significance and Impact of the Study: This is the first report regarding a lantibiotic gene cluster produced by Strep. thermophilus strain.

Original languageEnglish
Pages (from-to)641-649
Number of pages9
JournalJournal of Applied Microbiology
Volume110
Issue number3
DOIs
Publication statusPublished - 2011 Mar

Keywords

  • AII type
  • Bacteriocin
  • Immunity peptide
  • Lantibiotic
  • Operon
  • Streptococcus thermophilus

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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