Gene and protein expression of brain-derived neurotrophic factor and TrkB in bone and cartilage

T. Yamashiro; T. Fukunaga; K. Yamashita; N. Kobashi; T. Takano-Yamamoto

doi:10.1016/S8756-3282(01)00405-7

Gene and protein expression of brain-derived neurotrophic factor and TrkB in bone and cartilage

T. Yamashiro, T. Fukunaga, K. Yamashita, N. Kobashi, T. Takano-Yamamoto

Research output: Contribution to journal › Article › peer-review

51 Citations (Scopus)

Abstract

Gene and protein expressions of brain-derived neurotrophic factor (BDNF) and TrkB, the high-affinity receptor of BDNF, were investigated in the femur and mandibular condyle of rats by in situ hybridization and immunohistochemistry. BDNF and TrkB mRNA showed overlapped expression in chondrocytes in proliferating and mature zones of the epiphyseal growth plate cartilage and mandibular condylar cartilage, and in cuboidal-shaped active osteoblasts at the site of endochondral and intramembranous ossification and in trabecular bone. Expression of BDNF protein also showed a similar localization. The present study suggests that BDNF may participate in regulating the development and remodeling of bony tissue in the developing rat.

Original language	English
Pages (from-to)	404-409
Number of pages	6
Journal	Bone
Volume	28
Issue number	4
DOIs	https://doi.org/10.1016/S8756-3282(01)00405-7
Publication status	Published - 2001
Externally published	Yes

Keywords

Bone-derived neurotrophic factor (BDNF)
Chondrocyte
Immunohistochemistry
In situ hybridization
Osteoblast
TrkB

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Histology
Physiology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S8756-3282(01)00405-7

Cite this

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title = "Gene and protein expression of brain-derived neurotrophic factor and TrkB in bone and cartilage",

abstract = "Gene and protein expressions of brain-derived neurotrophic factor (BDNF) and TrkB, the high-affinity receptor of BDNF, were investigated in the femur and mandibular condyle of rats by in situ hybridization and immunohistochemistry. BDNF and TrkB mRNA showed overlapped expression in chondrocytes in proliferating and mature zones of the epiphyseal growth plate cartilage and mandibular condylar cartilage, and in cuboidal-shaped active osteoblasts at the site of endochondral and intramembranous ossification and in trabecular bone. Expression of BDNF protein also showed a similar localization. The present study suggests that BDNF may participate in regulating the development and remodeling of bony tissue in the developing rat.",

keywords = "Bone-derived neurotrophic factor (BDNF), Chondrocyte, Immunohistochemistry, In situ hybridization, Osteoblast, TrkB",

author = "T. Yamashiro and T. Fukunaga and K. Yamashita and N. Kobashi and T. Takano-Yamamoto",

note = "Funding Information: This study was supported by grants-in-aid (10470446, 10557197, 11771318) for scientific research from the Ministry of Education, Science and Culture of Japan.",

year = "2001",

doi = "10.1016/S8756-3282(01)00405-7",

language = "English",

volume = "28",

pages = "404--409",

journal = "Bone",

issn = "8756-3282",

publisher = "Elsevier Inc.",

number = "4",

}

TY - JOUR

T1 - Gene and protein expression of brain-derived neurotrophic factor and TrkB in bone and cartilage

AU - Yamashiro, T.

AU - Fukunaga, T.

AU - Yamashita, K.

AU - Kobashi, N.

AU - Takano-Yamamoto, T.

N1 - Funding Information: This study was supported by grants-in-aid (10470446, 10557197, 11771318) for scientific research from the Ministry of Education, Science and Culture of Japan.

PY - 2001

Y1 - 2001

N2 - Gene and protein expressions of brain-derived neurotrophic factor (BDNF) and TrkB, the high-affinity receptor of BDNF, were investigated in the femur and mandibular condyle of rats by in situ hybridization and immunohistochemistry. BDNF and TrkB mRNA showed overlapped expression in chondrocytes in proliferating and mature zones of the epiphyseal growth plate cartilage and mandibular condylar cartilage, and in cuboidal-shaped active osteoblasts at the site of endochondral and intramembranous ossification and in trabecular bone. Expression of BDNF protein also showed a similar localization. The present study suggests that BDNF may participate in regulating the development and remodeling of bony tissue in the developing rat.

AB - Gene and protein expressions of brain-derived neurotrophic factor (BDNF) and TrkB, the high-affinity receptor of BDNF, were investigated in the femur and mandibular condyle of rats by in situ hybridization and immunohistochemistry. BDNF and TrkB mRNA showed overlapped expression in chondrocytes in proliferating and mature zones of the epiphyseal growth plate cartilage and mandibular condylar cartilage, and in cuboidal-shaped active osteoblasts at the site of endochondral and intramembranous ossification and in trabecular bone. Expression of BDNF protein also showed a similar localization. The present study suggests that BDNF may participate in regulating the development and remodeling of bony tissue in the developing rat.

KW - Bone-derived neurotrophic factor (BDNF)

KW - Chondrocyte

KW - Immunohistochemistry

KW - In situ hybridization

KW - Osteoblast

KW - TrkB

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U2 - 10.1016/S8756-3282(01)00405-7

DO - 10.1016/S8756-3282(01)00405-7

M3 - Article

C2 - 11336921

AN - SCOPUS:0035032403

VL - 28

SP - 404

EP - 409

JO - Bone

JF - Bone

SN - 8756-3282

IS - 4

ER -

Gene and protein expression of brain-derived neurotrophic factor and TrkB in bone and cartilage

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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