Gene analysis of Cryptosporidium parvum HNJ-1 strain isolated in Japan

Masaaki Satoh; Isao Kimata; Motohiro Iseki; Yutaka Nakai

doi:10.1007/s00436-005-1474-8

Gene analysis of Cryptosporidium parvum HNJ-1 strain isolated in Japan

Masaaki Satoh, Isao Kimata, Motohiro Iseki, Yutaka Nakai

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

We analyzed genetically Cryptosporidium parvum HNJ-1 strain, which is the Japanese reference strain isolated from human in Japan. DNA sequences of genes for thrombospondin-related adhesive protein of Cryptosporidium-1 and Cryptosporidium-2 (TRAP-C1, TRAP-C2), heat shock protein 70 (HSP70), oocyst wall protein (COWP), beta-tubulin, alpha-tubulin, polythreonine-region (Poly-T), elongation factor 1 alpha (EF-1α), and 18S rRNA of this strain were determined. They showed high rate of homology to published sequences of genotype 2 strains, which were considered to be infective to both humans and animals. However, HNJ-1 had synonymous and non-synonymous substitutions in the nucleotide sequence of TRAP-C1 and beta-tubulin among HNJ-1 and published sequences of genotype 2 strains. These results implied that HNJ-1 strain was an unique subpopulation of genotype 2 strain of C. parvum.

Original language	English
Pages (from-to)	452-457
Number of pages	6
Journal	Parasitology Research
Volume	97
Issue number	6
DOIs	https://doi.org/10.1007/s00436-005-1474-8
Publication status	Published - 2005 Dec
Externally published	Yes

ASJC Scopus subject areas

Parasitology
veterinary(all)
Insect Science
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s00436-005-1474-8

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title = "Gene analysis of Cryptosporidium parvum HNJ-1 strain isolated in Japan",

abstract = "We analyzed genetically Cryptosporidium parvum HNJ-1 strain, which is the Japanese reference strain isolated from human in Japan. DNA sequences of genes for thrombospondin-related adhesive protein of Cryptosporidium-1 and Cryptosporidium-2 (TRAP-C1, TRAP-C2), heat shock protein 70 (HSP70), oocyst wall protein (COWP), beta-tubulin, alpha-tubulin, polythreonine-region (Poly-T), elongation factor 1 alpha (EF-1α), and 18S rRNA of this strain were determined. They showed high rate of homology to published sequences of genotype 2 strains, which were considered to be infective to both humans and animals. However, HNJ-1 had synonymous and non-synonymous substitutions in the nucleotide sequence of TRAP-C1 and beta-tubulin among HNJ-1 and published sequences of genotype 2 strains. These results implied that HNJ-1 strain was an unique subpopulation of genotype 2 strain of C. parvum.",

author = "Masaaki Satoh and Isao Kimata and Motohiro Iseki and Yutaka Nakai",

year = "2005",

month = dec,

doi = "10.1007/s00436-005-1474-8",

language = "English",

volume = "97",

pages = "452--457",

journal = "Parasitology Research",

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publisher = "Springer Verlag",

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T1 - Gene analysis of Cryptosporidium parvum HNJ-1 strain isolated in Japan

AU - Satoh, Masaaki

AU - Kimata, Isao

AU - Iseki, Motohiro

AU - Nakai, Yutaka

PY - 2005/12

Y1 - 2005/12

N2 - We analyzed genetically Cryptosporidium parvum HNJ-1 strain, which is the Japanese reference strain isolated from human in Japan. DNA sequences of genes for thrombospondin-related adhesive protein of Cryptosporidium-1 and Cryptosporidium-2 (TRAP-C1, TRAP-C2), heat shock protein 70 (HSP70), oocyst wall protein (COWP), beta-tubulin, alpha-tubulin, polythreonine-region (Poly-T), elongation factor 1 alpha (EF-1α), and 18S rRNA of this strain were determined. They showed high rate of homology to published sequences of genotype 2 strains, which were considered to be infective to both humans and animals. However, HNJ-1 had synonymous and non-synonymous substitutions in the nucleotide sequence of TRAP-C1 and beta-tubulin among HNJ-1 and published sequences of genotype 2 strains. These results implied that HNJ-1 strain was an unique subpopulation of genotype 2 strain of C. parvum.

AB - We analyzed genetically Cryptosporidium parvum HNJ-1 strain, which is the Japanese reference strain isolated from human in Japan. DNA sequences of genes for thrombospondin-related adhesive protein of Cryptosporidium-1 and Cryptosporidium-2 (TRAP-C1, TRAP-C2), heat shock protein 70 (HSP70), oocyst wall protein (COWP), beta-tubulin, alpha-tubulin, polythreonine-region (Poly-T), elongation factor 1 alpha (EF-1α), and 18S rRNA of this strain were determined. They showed high rate of homology to published sequences of genotype 2 strains, which were considered to be infective to both humans and animals. However, HNJ-1 had synonymous and non-synonymous substitutions in the nucleotide sequence of TRAP-C1 and beta-tubulin among HNJ-1 and published sequences of genotype 2 strains. These results implied that HNJ-1 strain was an unique subpopulation of genotype 2 strain of C. parvum.

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JO - Parasitology Research

JF - Parasitology Research

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IS - 6

ER -

Gene analysis of Cryptosporidium parvum HNJ-1 strain isolated in Japan

Abstract

ASJC Scopus subject areas

UN SDGs

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