Gellan sulfate inhibits plasmodium falciparum growth and invasion of red blood cells in vitro

Frances Cagayat Recuenco, Kyousuke Kobayashi, Akiko Ishiwa, Yukiko Enomoto-Rogers, Noreen Grace V. Fundador, Tatsuki Sugi, Hitoshi Takemae, Tatsuya Iwanaga, Fumi Murakoshi, Haiyan Gong, Atsuko Inomata, Taisuke Horimoto, Tadahisa Iwata, Kentaro Kato

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Here, we assessed the sulfated derivative of the microbial polysaccharide gellan gum and derivatives of λ and K-carrageenans for their ability to inhibit Plasmodium falciparum 3D7 and Dd2 growth and invasion of red blood cells in vitro. Growth inhibition was assessed by means of flow cytometry after a 96-h exposure to the inhibitors and invasion inhibition was assessed by counting ring parasites after a 20-h exposure to them. Gellan sulfate strongly inhibited invasion and modestly inhibited growth for both P. falciparum 3D7 and Dd2; both inhibitory effects exceeded those achieved with native gellan gum. The hydrolyzed λ-carrageenan and oversulfated k-carrageenan were less inhibitory than their native forms. In vitro cytotoxicity and anticoagulation assays performed to determine the suitability of the modified polysaccharides for in vivo studies showed that our synthesized gellan sulfate had low cytotoxicity and anticoagulant activity.

Original languageEnglish
Article number4723
JournalScientific reports
Volume4
DOIs
Publication statusPublished - 2014 Apr 17
Externally publishedYes

ASJC Scopus subject areas

  • General

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