Gelatin-Polyaniline Composite Nanofibers Enhanced Excitation-Contraction Coupling System Maturation in Myotubes

Serge Ostrovidov, Majid Ebrahimi, Hojae Bae, Hung Kim Nguyen, Sahar Salehi, Sang Bok Kim, Akichika Kumatani, Tomokazu Matsue, Xuetao Shi, Ken Nakajima, Shizu Hidema, Makoto Osanai, Ali Khademhosseini

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

In this study, composite gelatin-polyaniline (PANI) nanofibers doped with camphorsulfonic acid (CSA) were fabricated by electrospinning and used as substrates to culture C2C12 myoblast cells. We observed enhanced myotube formation on composite gelatin-PANI nanofibers compared to gelatin nanofibers, concomitantly with enhanced myotube maturation. Thus, in myotubes, intracellular organization, colocalization of the dihydropyridine receptor (DHPR) and ryanodine receptor (RyR), expression of genes correlated to the excitation-contraction (E-C) coupling apparatus, calcium transients, and myotube contractibility were increased. Such composite material scaffolds combining topographical and electrically conductive cues may be useful to direct skeletal muscle cell organization and to improve cellular maturation, functionality, and tissue formation.

Original languageEnglish
Pages (from-to)42444-42458
Number of pages15
JournalACS Applied Materials and Interfaces
Volume9
Issue number49
DOIs
Publication statusPublished - 2017 Dec 13

Keywords

  • C2C12
  • composite nanofibers
  • excitationâ'contraction coupling
  • gelatin
  • intracellular calcium
  • myotube maturation
  • polyaniline

ASJC Scopus subject areas

  • Materials Science(all)

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