Gel sheet based skeletal muscle cell culture system integrated with the microelectrode array device

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

We developed the fibrin gel-based C2C12 myotube culture system integrated with the microelectrode array device to afford a physiologically relevant and stable bioassay system. Myotube/fibrin gel culture system was prepared by transferring a myotube monolayer from a glass substrate to a fibrin gel while retaining the original line patterns of myotubes. By attaching the myotube/fibrin gel onto the microelectrode array, we demonstrated site-specific electrical pulse stimulation of each myotube line pattern. This device would enable highly-accurate bioassay of contraction-mediated glucose metabolic alterations in myotubes by patterning test and control cells stimulated with different contraction/exercise stress next to each other.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages175-177
Number of pages3
Publication statusPublished - 2010 Dec 1
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: 2010 Oct 32010 Oct 7

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume1

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CountryNetherlands
CityGroningen
Period10/10/310/10/7

Keywords

  • Cell transfer
  • Fibrin gel
  • Microelectrode arrays
  • Skeletal muscle cell
  • Type 2 diabetes

ASJC Scopus subject areas

  • Control and Systems Engineering

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