TY - GEN
T1 - Gel sheet based skeletal muscle cell culture system integrated with the microelectrode array device
AU - Nagamine, Kuniaki
AU - Kaji, Hirokazu
AU - Kanzaki, Makoto
AU - Nishizawa, Matsuhiko
PY - 2010/12/1
Y1 - 2010/12/1
N2 - We developed the fibrin gel-based C2C12 myotube culture system integrated with the microelectrode array device to afford a physiologically relevant and stable bioassay system. Myotube/fibrin gel culture system was prepared by transferring a myotube monolayer from a glass substrate to a fibrin gel while retaining the original line patterns of myotubes. By attaching the myotube/fibrin gel onto the microelectrode array, we demonstrated site-specific electrical pulse stimulation of each myotube line pattern. This device would enable highly-accurate bioassay of contraction-mediated glucose metabolic alterations in myotubes by patterning test and control cells stimulated with different contraction/exercise stress next to each other.
AB - We developed the fibrin gel-based C2C12 myotube culture system integrated with the microelectrode array device to afford a physiologically relevant and stable bioassay system. Myotube/fibrin gel culture system was prepared by transferring a myotube monolayer from a glass substrate to a fibrin gel while retaining the original line patterns of myotubes. By attaching the myotube/fibrin gel onto the microelectrode array, we demonstrated site-specific electrical pulse stimulation of each myotube line pattern. This device would enable highly-accurate bioassay of contraction-mediated glucose metabolic alterations in myotubes by patterning test and control cells stimulated with different contraction/exercise stress next to each other.
KW - Cell transfer
KW - Fibrin gel
KW - Microelectrode arrays
KW - Skeletal muscle cell
KW - Type 2 diabetes
UR - http://www.scopus.com/inward/record.url?scp=84884340042&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84884340042&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84884340042
SN - 9781618390622
T3 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
SP - 175
EP - 177
BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
T2 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Y2 - 3 October 2010 through 7 October 2010
ER -