GATA Transcription Factors Negatively Regulate Erythropoietin Gene Expression

Shigehiko Imagawa, Masayuki Yamamoto, Yasusada Miura

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

We examined regulation of the human erythropoietin (Epo) gene through the GATA sequence in the Epo promoter, and demonstrated that Hep3B and HepG2 cells express human GATA-2 (hGATA-2) mRNA and protein. Nuclear extracts of QT6 cells transfected with hGATA-1, -2 or -3 transcription factors revealed specific binding to the GATA element in the human Epo gene promoter by gel mobility shift assay. Transient transfection of Hep3B cells with hGATA-1, -2 or -3 demonstrated that each of these transcription factors significantly decreased the level of expression of Epo mRNA as assessed by a competitive polymerase chain reaction. Furthermore, transient transfection of Hep3B cells with hGATA-1, -2 and -3 and an Epo reporter gene construct showed significant inhibition of the Epo promoter. We conclude that the hGATA-1, -2 and -3 transcription factors specifically bind to the GATA element in the human Epo gene promoter and negatively regulate Epo gene expression.

Original languageEnglish
Pages (from-to)248-256
Number of pages9
JournalActa Haematologica
Volume95
Issue number3-4
DOIs
Publication statusPublished - 1996 Jan 1

Keywords

  • Erythropoietin gene expression
  • GATA transcription factor
  • Promoter
  • Reporter gene
  • Transient transfection

ASJC Scopus subject areas

  • Hematology

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