GATA-1 transcription is controlled by distinct regulatory mechanisms during primitive and definitive erythropoiesis

Ko Onodera, Satoru Takahashi, Shigeko Nishimura, Jun Ohta, Hozumi Motohashi, Kentaro Yomogida, Norio Hayashi, James Douglas Engel, Masayuki Yamamoto

Research output: Contribution to journalArticlepeer-review

141 Citations (Scopus)

Abstract

Transcription factor GATA-1 is required for the terminal differentiation of both the primitive and definitive erythroid cell lineages, and yet the regulatory mechanisms of GATA-1 itself are not well understood. To clarify how the GATA-1 gene is transcriptionally controlled in vivo, presumptive regulatory regions of the gene were tested by fusion to a reporter gene and then examined in transgenic mice. We found that a transcriptional control element located between -3.9 and -2.6 kb 5' to the erythroid first exon serves as an activating element and that this sequence alone is sufficient to recapitulate the expression of GATA-1 (but uniquely in primitive erythroid cells). Addition of sequences from the GATA-1 first intron to this upstream element provides a necessary and sufficient condition for complete recapitulation of GATA-1 expression in both primitive and definitive erythroid cells. The first intron element does not possess intrinsic transcriptional activation potential when linked to the GATA- gene promoter but rather requires the upstream activating element fur its activity. These experiments show that GATA-1 gene expression is regulated by discrete transcriptional control elements during definitive and primitive erythropniesis: The 5' element displays properties anticipated for a primitive erythrnid cell-specific activating element, and the novel element within the GATA-1 first intron specifically augments this activity in definitive erythroid cells.

Original languageEnglish
Pages (from-to)4487-4492
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number9
DOIs
Publication statusPublished - 1997 Apr 29

ASJC Scopus subject areas

  • General

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