GATA-1 testis activation region is essential for Sertoli cell-specific expression of GATA-1 gene in transgenic mouse

Junko Wakabayashi, Kentaro Yomogida, Osamu Nakajima, Keigyou Yoh, Satoru Takahashi, James Douglas Engel, Kinuko Ohneda, Masayuki Yamamoto

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background: The erythroid transcription factor GATA-1 is also expressed in Sertoli cells of the testis. The testicular expression of GATA-1 is regulated in a developmental and spermatogenic stage-specific manner.To further clarify the regulatory mechanisms of testicular GATA-1 gene expression, we carried out transgenic reporter gene expression analyses. Results: We found that GATA-1 expression in Sertoli cells is markedly decreased concomitant with the emergence of elongated spermatids in the seminiferous tubules. Transgenic reporter mouse analyses revealed that a 15 kb GATA-1 genomic region is sufficient to recapitulate the gene expression profile in Sertoli cells. While the GATA-1 haematopoietic enhancer and the proximal first exon are included within the 15 kb genomic region, these regulatory elements are not essential for GATA-1 expression in Sertoli cells. Further analyses using deletion constructs revealed that a 1.5 kb region 5′ to the GATA-1 haematopoietic enhancer is essential for gene expression in Sertoli cells and this region is referred to as the GATA-1 testis activation region. Conclusion: These results thus demonstrated that the GATA-1 testis activation region is essential for Sertoli cell-specific expression of GATA-1 gene. The 15 kb genomic region is applicable and useful for the expression vector system specific for adult Sertoli cells in stage VII to IX.

Original languageEnglish
Pages (from-to)619-630
Number of pages12
JournalGenes to Cells
Volume8
Issue number7
DOIs
Publication statusPublished - 2003 Jul 1
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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