GATA-1 is required for expression of FcεRI on mast cells: Analysis of mast cells derived from GATA-1 knockdown mouse bone marrow

Chiharu Nishiyama, Tomonobu Ito, Makoto Nishiyama, Shigehiro Masaki, Keiko Maeda, Nobuhiro Nakano, William Ng, Kanako Fukuyama, Masayuki Yamamoto, Ko Okumura, Hideoki Ogawa

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The high-affinity receptor for IgE (FcεRI) that is expressed on the surface of mast cells plays an important role in antigen/IgE-mediated allergic reactions. We have previously found that critical elements in the promoter of the FcεRI α- and β-chain genes are recognized by the transcription factor GATA-1 in electrophoretic mobility shift assays coupled with a transient expression system for the α- and β-chain promoters. To confirm that GATA-1 is involved in the expression of FcεRI definitively, we generated bone marrow-derived mast cells from GATA-1 knockdown (KD) heterozygous mice. FACS analysis showed that the frequency of FcεRI-positive cells was significantly decreased in mast cells derived from bone marrow of GATA-1 KD mice. Reverse transcription-PCR analysis showed that the level of transcripts not only for GATA-1 but also for both the α- and β-chains was significantly lower in KD mast cells, whereas that of the FcεRI γ-chain was not affected. Degranulation caused by cross-linking of FcεRI on mast cells prepared from KD mice was markedly repressed in comparison with that of wild-type mast cells. We concluded that the transcription factor GATA-1 positively regulates FcεRI α- and β-chain expression and therefore is involved in mast cell development.

Original languageEnglish
Pages (from-to)847-856
Number of pages10
JournalInternational immunology
Volume17
Issue number7
DOIs
Publication statusPublished - 2005 Jul 1
Externally publishedYes

Keywords

  • GATA-1
  • Knockdown mouse
  • Mast cells
  • Transcription factor

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Nishiyama, C., Ito, T., Nishiyama, M., Masaki, S., Maeda, K., Nakano, N., Ng, W., Fukuyama, K., Yamamoto, M., Okumura, K., & Ogawa, H. (2005). GATA-1 is required for expression of FcεRI on mast cells: Analysis of mast cells derived from GATA-1 knockdown mouse bone marrow. International immunology, 17(7), 847-856. https://doi.org/10.1093/intimm/dxh278