Gas6 regulates mesangial cell proliferation through Axl in experimental glomerulonephritis

Motoko Yanagita, Hidenori Arai, Kenji Ishii, Toru Nakano, Kazumasa Ohashi, Kensaku Mizuno, Brian Varnum, Atsushi Fukatsu, Toshio Doi, Toru Kita

Research output: Contribution to journalArticlepeer-review

85 Citations (Scopus)

Abstract

Proliferation of mesangial cells is a hallmark of glomerular disease, and understanding its regulatory mechanism is clinically important. Previously, we demonstrated that the product of growth arrest-specific gene 6 (Gas6) stimulates mesangial cell proliferation through binding to its cell-surface receptor Axl in vitro. We also showed that warfarin and the extracellular domain of Axl conjugated with Fc portion of human IgG1 (Axl-Fc) inhibit mesangial cell proliferation by interfering the Gas6/Axl pathway in vitro. In the present study, therefore, we examined in vivo roles of Gas6 and Axl in an experimental model of mesangial proliferative glomerulonephritis induced by the injection of anti-Thy1.1 antibody (Thy1 GN). In Thy1 GN, expression of Gas6 and Axl was markedly increased in glomeruli, and paralleled the progression of mesangial cell proliferation. Administration of warfarin or daily injection of Axl-Fc inhibited mesangial cell proliferation, and abolished the induction of platelet-derived growth factor-B mRNA and protein in Thy1 GN. Moreover, the anti-proliferative effect of warfarin was achieved at lower concentrations than those in routine clinical use. These findings indicate that the Gas6/Axl pathway plays a key role in mesangial cell proliferation in vivo, and could be a potentially important therapeutic target for the treatment of renal disease.

Original languageEnglish
Article number64093
Pages (from-to)1423-1432
Number of pages10
JournalAmerican Journal of Pathology
Volume158
Issue number4
DOIs
Publication statusPublished - 2001

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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