Abstract
Gas-1iquid Interfacial atmospheric-pressure plasma jets (GLI-APPJ) are used medically for plasma-induced cell-membrane permeabilization. In an attempt to identify the dominant factors induced by GLI-APPJ responsible for enhancing cell-membrane permeability, the concentration and distribution of plasma-produced reactive species in the gas and liquid phase regions are measured. These reactive species are classified in terms of their life-span: long-lived (e.g., H2O2), short-lived (e.g., O2•-), and extremely-short-lived (e.g., •OH). The concentration of plasma-produced •OHaq in the liquid phase region decreases with an increase in solution thickness (<1 mm), and plasma-induced cell-membrane permeabilization is found to decay markedly as the thickness of the solution increases. Furthermore, the horizontally center-localized distribution of •OHaq, resulting from the center-peaked distribution of •OH in the gas phase region, corresponds with the distribution of the perme-abilized cells upon APPJ irradiation, whereas the overall plasma-produced oxidizing species such as H2O2aq in solution exhibit a doughnut-shaped horizontal distribution. These results suggest that •OHaq is likely one of the dominant factors responsible for plasma-induced cell-membrane permeabilization.
Original language | English |
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Pages (from-to) | 3-11 |
Number of pages | 9 |
Journal | Journal of Clinical Biochemistry and Nutrition |
Volume | 60 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2017 Jan |
Keywords
- Atmospheric pressure plasma jet
- Cell membrane permeabilization
- Gas liquid interfacial plasma
- Plasma medicine
- Reactive species
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Nutrition and Dietetics
- Clinical Biochemistry