TY - JOUR
T1 - Garcinone B reduces prostaglandin E2 release and NF-κB-mediated transcription in C6 rat glioma cells
AU - Yamakuni, Tohru
AU - Aoki, Koichi
AU - Nakatani, Keigo
AU - Kondo, Nobuhiko
AU - Oku, Hisae
AU - Ishiguro, Kyoko
AU - Ohizumi, Yasushi
N1 - Funding Information:
This work was partly supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sport and Culture of Japan. We thank Dr. H. Inoue, National Cardiovascular Center Research Institute, for their helpful discussion and Dr. J. Fujisawa, Kansai Medical University, for providing an NF-κB promoter construct.
PY - 2006/2/20
Y1 - 2006/2/20
N2 - In the course of our survey of natural compounds inhibiting prostaglandin E2 release and/or lipopolysaccharide (LPS)-induced transcriptional stimulation via NF-κB, a central regulator of inflammatory genes, from natural resources, we found garcinone B, a xanthone from callus tissue culture of Hypericum patulum, as a compound with such pharmacological activities, that is a derivative of γ-mangostin which potently inhibits COX-1 and COX-2 activities to reduce PGE2 release from C6 rat glioma cells, and inhibits IKK activity to prevent NF-κB-dependent COX-2 gene transcription. Garcinone B, to a lesser extent, reduced A23187-induced increase in prostaglandin E2 release than γ-mangostin and its structurally related compound, patulone, in C6 cells. This compound also prevented LPS-induced stimulation of NF-κB-dependent transcription. These results suggest that garcinone B becomes a unique pharmacological tool to investigate intracellular signaling pathways involved in inflammation.
AB - In the course of our survey of natural compounds inhibiting prostaglandin E2 release and/or lipopolysaccharide (LPS)-induced transcriptional stimulation via NF-κB, a central regulator of inflammatory genes, from natural resources, we found garcinone B, a xanthone from callus tissue culture of Hypericum patulum, as a compound with such pharmacological activities, that is a derivative of γ-mangostin which potently inhibits COX-1 and COX-2 activities to reduce PGE2 release from C6 rat glioma cells, and inhibits IKK activity to prevent NF-κB-dependent COX-2 gene transcription. Garcinone B, to a lesser extent, reduced A23187-induced increase in prostaglandin E2 release than γ-mangostin and its structurally related compound, patulone, in C6 cells. This compound also prevented LPS-induced stimulation of NF-κB-dependent transcription. These results suggest that garcinone B becomes a unique pharmacological tool to investigate intracellular signaling pathways involved in inflammation.
KW - C6 rat glioma cells
KW - Garcinone B
KW - NF-κB-dependent transcription
KW - Prostaglandin E release
KW - Xanthones
KW - γ-Mangostin
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U2 - 10.1016/j.neulet.2005.10.023
DO - 10.1016/j.neulet.2005.10.023
M3 - Article
C2 - 16260090
AN - SCOPUS:31344441761
VL - 394
SP - 206
EP - 210
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 3
ER -