Garcinone B reduces prostaglandin E2 release and NF-κB-mediated transcription in C6 rat glioma cells

Tohru Yamakuni, Koichi Aoki, Keigo Nakatani, Nobuhiko Kondo, Hisae Oku, Kyoko Ishiguro, Yasushi Ohizumi

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

In the course of our survey of natural compounds inhibiting prostaglandin E2 release and/or lipopolysaccharide (LPS)-induced transcriptional stimulation via NF-κB, a central regulator of inflammatory genes, from natural resources, we found garcinone B, a xanthone from callus tissue culture of Hypericum patulum, as a compound with such pharmacological activities, that is a derivative of γ-mangostin which potently inhibits COX-1 and COX-2 activities to reduce PGE2 release from C6 rat glioma cells, and inhibits IKK activity to prevent NF-κB-dependent COX-2 gene transcription. Garcinone B, to a lesser extent, reduced A23187-induced increase in prostaglandin E2 release than γ-mangostin and its structurally related compound, patulone, in C6 cells. This compound also prevented LPS-induced stimulation of NF-κB-dependent transcription. These results suggest that garcinone B becomes a unique pharmacological tool to investigate intracellular signaling pathways involved in inflammation.

Original languageEnglish
Pages (from-to)206-210
Number of pages5
JournalNeuroscience Letters
Volume394
Issue number3
DOIs
Publication statusPublished - 2006 Feb 20

Keywords

  • C6 rat glioma cells
  • Garcinone B
  • NF-κB-dependent transcription
  • Prostaglandin E release
  • Xanthones
  • γ-Mangostin

ASJC Scopus subject areas

  • Neuroscience(all)

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    Yamakuni, T., Aoki, K., Nakatani, K., Kondo, N., Oku, H., Ishiguro, K., & Ohizumi, Y. (2006). Garcinone B reduces prostaglandin E2 release and NF-κB-mediated transcription in C6 rat glioma cells. Neuroscience Letters, 394(3), 206-210. https://doi.org/10.1016/j.neulet.2005.10.023