Galectins in the abdominal cavity of the conger eel Conger myriaster participate in the cellular encapsulation of parasitic nematodes by host cells

Osamu Nakamura, Mizuki Watanabe, Tomohisa Ogawa, Koji Muramoto, Kazuo Ogawa, Shigeyuki Tsutsui, Hisao Kamiya

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    16 Citations (Scopus)

    Abstract

    Congerin is a proto-type galectin distributed on the skin and mucosal epithelia of the upper digestive tract of the Japanese conger eel Conger myriaster. It has at least 2 isotypes, namely, congerin I and II, and plays a role in bio-defense at the body surface. In the current study, we identified both isotypes in the peritoneal fluid and peritoneal cells of C. myriaster by western blot and mass spectrometry (MS)/MS analysis. Cucullanus nematodes parasitize the abdominal cavity of C. myriaster, and immunohistochemical analyses demonstrated that congerins can bind to both the body surface of the encapsulated nematodes and the encapsulating cells. Furthermore, adhesion of the peritoneal cells to Sepharose particles was greatly accelerated when the microspheres were coated with congerin. Indeed, this effect was significantly hampered by the addition of lactose. These results indicate that congerin participates in the cellular encapsulation of the Cucullanus nematode via the induction of cellular adhesion to the parasites depending on lectin-glycoside recognition.

    Original languageEnglish
    Pages (from-to)780-787
    Number of pages8
    JournalFish and Shellfish Immunology
    Volume33
    Issue number4
    DOIs
    Publication statusPublished - 2012 Oct

    Keywords

    • Cellular encapsulation
    • Conger eel
    • Congerin
    • Galectin
    • Nematode

    ASJC Scopus subject areas

    • Environmental Chemistry
    • Aquatic Science

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