GABAergic local interneurons shape female fruit fly response to mating songs

Daichi Yamada, Hiroshi Ishimoto, Xiaodong Li, Tsunehiko Kohashi, Yuki Ishikawa, Azusa Kamikouchi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Many animals use acoustic signals to attract a potential mating partner. In fruit flies (Drosophila melanogaster), the courtship pulse song has a species-specific interpulse interval (IPI) that activates mating. Although a series of auditory neurons in the fly brain exhibit different tuning patterns to IPIs, it is unclear how the response of each neuron is tuned. Here, we studied the neural circuitry regulating the activity of antennal mechanosensory and motor center (AMMC)-B1 neurons, key secondary auditory neurons in the excitatory neural pathway that relay song information. By performing Ca2+ imaging in female flies, we found that the IPI selectivity observed in AMMC-B1 neurons differs from that of upstream auditory sensory neurons [Johnston’s organ (JO)-B]. Selective knock-down of a GABAA receptor subunit in AMMC-B1 neurons increased their response to short IPIs, suggesting that GABA suppresses AMMC-B1 activity at these IPIs. Connection mapping identified two GABAergic local interneurons that synapse with AMMC-B1 and JO-B. Ca2+ imaging combined with neuronal silencing revealed that these local interneurons, AMMC-LN and AMMC-B2, shape the response pattern of AMMC-B1 neurons at a 15 ms IPI. Neuronal silencing studies further suggested that both GABAergic local interneurons suppress the behavioral response to artificial pulse songs in flies, particularly those with a 15 ms IPI. Altogether, we identified a circuit containing two GABAergic local interneurons that affects the temporal tuning of AMMC-B1 neurons in the song relay pathway and the behavioral response to the courtship song. Our findings suggest that feedforward inhibitory pathways adjust the behavioral response to courtship pulse songs in female flies.

Original languageEnglish
Pages (from-to)4329-4347
Number of pages19
JournalJournal of Neuroscience
Volume38
Issue number18
DOIs
Publication statusPublished - 2018 May 2
Externally publishedYes

Keywords

  • Auditory neurons
  • Courtship song
  • Drosophila melanogaster
  • Feedforward inhibition
  • Neural circuit

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'GABAergic local interneurons shape female fruit fly response to mating songs'. Together they form a unique fingerprint.

  • Cite this