GABAA receptor subunit β1 is involved in the formation of protease-resistant prion protein in prion-infected neuroblastoma cells

Tomohiro Kimura, Kensuke Ishikawa, Yuji Sakasegawa, Kenta Teruya, Tetsutaro Sata, Hermann Schätzl, Katsumi Doh-ura

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

γ-Aminobutyric acid type A (GABAA) receptor β1 (gabrb1), a subunit of GABAA receptors involved in inhibitory effects on neurotransmission, was found to associate with the formation of protease-resistant prion protein in prion-infected neuroblastoma cells. Silencing of gabrb1 gene expression significantly decreased the abnormal prion protein level but paradoxically increased the normal prion protein level. Treatment with a gabrb1-specific inhibitor, salicylidene salicylhydrazide, dose-dependently decreased the abnormal prion protein level, but silencing of other GABAA receptor subunits' gene expression and treatments with the receptor antagonists and agonists did not. Therefore, gabrb1 involvement in abnormal prion protein formation is independent of GABAA receptors.

Original languageEnglish
Pages (from-to)1193-1198
Number of pages6
JournalFEBS Letters
Volume584
Issue number6
DOIs
Publication statusPublished - 2010 Mar

Keywords

  • Antagonist
  • GABAA receptor
  • Gabrb1
  • Prion
  • SiRNA

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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