G1 cyclins link proliferation, pluripotency and differentiation of embryonic stem cells

Lijun Liu, Wojciech Michowski, Hiroyuki Inuzuka, Kouhei Shimizu, Naoe Taira Nihira, Joel M. Chick, Na Li, Yan Geng, Alice Y. Meng, Alban Ordureau, Aleksandra Kołodziejczyk, Keith L. Ligon, Roderick T. Bronson, Kornelia Polyak, J. Wade Harper, Steven P. Gygi, Wenyi Wei, Piotr Sicinski

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)

Abstract

Progression of mammalian cells through the G1 and S phases of the cell cycle is driven by the D-type and E-type cyclins. According to the current models, at least one of these cyclin families must be present to allow cell proliferation. Here, we show that several cell types can proliferate in the absence of all G1 cyclins. However, following ablation of G1 cyclins, embryonic stem (ES) cells attenuated their pluripotent characteristics, with the majority of cells acquiring the trophectodermal cell fate. We established that G1 cyclins, together with their associated cyclin-dependent kinases (CDKs), phosphorylate and stabilize the core pluripotency factors Nanog, Sox2 and Oct4. Treatment of murine ES cells, patient-derived glioblastoma tumour-initiating cells, or triple-negative breast cancer cells with a CDK inhibitor strongly decreased Sox2 and Oct4 levels. Our findings suggest that CDK inhibition might represent an attractive therapeutic strategy by targeting glioblastoma tumour-initiating cells, which depend on Sox2 to maintain their tumorigenic potential.

Original languageEnglish
Pages (from-to)177-188
Number of pages12
JournalNature cell biology
Volume19
Issue number3
DOIs
Publication statusPublished - 2017 Mar 1

ASJC Scopus subject areas

  • Cell Biology

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