FUT-175 as a potent inhibitor of C5/C3 convertase activity for production of C5a and C3a

Reiko Inagi, Toshio Miyata, Kenji Maeda, Satoshi Sugiyama, Akio Miyama, Izumi Nakashima

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

We examined the inhibitory effect of FUT-175 on the C3/C5 convertase activity of the cobra venom factor-derived enzyme CVF,Bb by measuring C5b6-mediated reactive lysis of unsensitized guinea pig erythrocytes and by measuring directly the released fragments C3-des-Arg and C5a-des-Arg. In this study, we showed that the concentration of 4.5 × 10-6 M of FUT-175 caused 50% inhibition of C5 convertase activity of CVF,Bb in reactive hemolysis assays, and that 4.0×10-6 M FUT-175 caused 50% inhibition of the production of C3a and C5a generated by the C3/C5 convertase activity of CVF,Bb.

Original languageEnglish
Pages (from-to)49-52
Number of pages4
JournalImmunology Letters
Volume27
Issue number1
DOIs
Publication statusPublished - 1991 Jan
Externally publishedYes

Keywords

  • C3/C5 convertase
  • C3a-des-Arg
  • C5a-des-Arg
  • CVF,Bb
  • Protease inhibitor

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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