Examination of the sequence of a hypothetical gene with an unknown function included in the carotenogenic gene cluster in the genome of a thermoacidophilic archaeon Sulfolobus solfataricus led to the prediction that the gene encodes a novel-type lycopene β-cyclase, whose N- and C-terminal halves are homologous to the subunits of the bacterial heterodimeric enzymes. The recombinant expression of the gene in lycopene-producing Escherichia coli resulted in the accumulation of β-carotene in the cells, which verifies the function of the gene. Homologues of the archaeal lycopene β-cyclase from various organisms such as bacteria, archaea, and fungi have been reported. Although their primary structures are clearly specific to the biological taxa, a phylogenetic analysis revealed the unexpected complicity of the evolutional route of these enzymes.
|Number of pages||6|
|Journal||Biochemical and biophysical research communications|
|Publication status||Published - 2003 Jun 6|
- Lycopene β-cyclase
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology