Fusion of an intact secretory protein permits a misfolded protein to exit from the endoplasmic reticulum in yeast

Kengo Suyama, Mizue Hori, Katsuya Gomi, Takahiro Shintani

    Research output: Contribution to journalArticlepeer-review

    1 Citation (Scopus)

    Abstract

    Upon exit from the endoplasmic reticulum (ER), the nascent polypeptides of secretory proteins undergo sorting events. If properly folded, they are directly or indirectly recognized by the coat proteins of budding vesicles for forward transport, while unfolded or misfolded proteins are retained in the ER by a quality control mechanism. To gain insight into the interplay between ER export and ER quality control, we fused a secretory protein invertase to the C-terminus of mutated carboxypeptidase Y (CPY∗), a model ER-associated degradation (ERAD) substrate in Saccharomyces cerevisiae. This substrate, designated CPY∗-Inv, was largely exported from the ER, although it was fully recognized by the ERAD-related lectin, Yos9, and hence degraded by the ERAD when it remained in the ER. CPY∗-Inv relied primarily on the p24 complex, a putative ER export receptor for invertase, for escape from ERAD, suggesting that the ERAD and the ER export of soluble secretory proteins are competitive.

    Original languageEnglish
    Pages (from-to)49-59
    Number of pages11
    JournalBioscience, Biotechnology and Biochemistry
    Volume78
    Issue number1
    DOIs
    Publication statusPublished - 2014

    Keywords

    • Carboxypeptidase Y
    • Endoplasmic reticulum (ER)-associated degradation
    • Invertase
    • Misfolded protein
    • Yeast

    ASJC Scopus subject areas

    • Biotechnology
    • Analytical Chemistry
    • Biochemistry
    • Applied Microbiology and Biotechnology
    • Molecular Biology
    • Organic Chemistry

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