Fusion of an intact secretory protein permits a misfolded protein to exit from the endoplasmic reticulum in yeast

Kengo Suyama, Mizue Hori, Katsuya Gomi, Takahiro Shintani

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Upon exit from the endoplasmic reticulum (ER), the nascent polypeptides of secretory proteins undergo sorting events. If properly folded, they are directly or indirectly recognized by the coat proteins of budding vesicles for forward transport, while unfolded or misfolded proteins are retained in the ER by a quality control mechanism. To gain insight into the interplay between ER export and ER quality control, we fused a secretory protein invertase to the C-terminus of mutated carboxypeptidase Y (CPY∗), a model ER-associated degradation (ERAD) substrate in Saccharomyces cerevisiae. This substrate, designated CPY∗-Inv, was largely exported from the ER, although it was fully recognized by the ERAD-related lectin, Yos9, and hence degraded by the ERAD when it remained in the ER. CPY∗-Inv relied primarily on the p24 complex, a putative ER export receptor for invertase, for escape from ERAD, suggesting that the ERAD and the ER export of soluble secretory proteins are competitive.

Original languageEnglish
Pages (from-to)49-59
Number of pages11
JournalBioscience, Biotechnology and Biochemistry
Volume78
Issue number1
DOIs
Publication statusPublished - 2014 Jan 1

Keywords

  • Carboxypeptidase Y
  • Endoplasmic reticulum (ER)-associated degradation
  • Invertase
  • Misfolded protein
  • Yeast

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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